[Histonet] Hemathoxylin free-floating

[Pablo Sanchez-Quinteiro]

Dear Histonetters,

I have free floating either 30 or 90 microns thick cryostat sections, 
which I have immunolabelled and studied at the confocal microsope.

Now I would like to counterstain them with Hematoxylin & Eosin always 
free-floating.

I would be very grateful if you could give me a procedure.

Cheers

Pablo Sanchez-Quinteiro
Facuty of Veterinary Sciences
27002 Lugo
SPAIN