[Histonet] PAS/D digestion problem

[Rene J Buesa]

John:
  I always used successfully the following procedure:
   
  a- prepare a "diastase buffer": anhydrous sodium chloride (8 g) + anhydrous dibasic sodium phosphate (0.282 g) + anhydrous monobasic sodium phosphate (1.974 g) + distilled water UP TO 1 liter. This buffer will have a pH @ 6 and has to be kept in the refrigerator.
   
  b- glycogen digesting solution: take 40 mL of the buffer and heat it to 37ºC and add 0.5 g of diastase of malt (Fisher brand "maltin" cat.No. D22-100).
   
  c- incubate the hydrated section at 37ºC for 30 minutes
   
  Proceed with the PAS, counter stain, etc., etc.
  René J.
  

Josh Wray <jwray78 <@t> gmail.com> wrote:
  
 

 __________________________________________________
Do You Yahoo!?
Tired of spam?  Yahoo! Mail has the best spam protection around 
http://mail.yahoo.com