[Histonet] Ki-67 for mouse

[Jacqui Detmar]

Hi there.  Re: this clone on mouse FFPE tissues, I find that I need to
do citrate buffer retrieval in a microwave pressure cooker in order to
see binding.  Do you find this to be true for you, too?  If not, what
method of antigen retrieval do you use?  I find the pressure cooker
method makes my sections look crappy...poor nuclear counterstaining....


Jacqui

Jacqui Detmar, Post-doctoral Fellow
Samuel Lunenfeld Research Institute, room 876
Mount Sinai Hospital
600 University Avenue
Toronto, ON, Canada
M5G 1X5

phone:   416-586-4800 x2451/x2290
fax:        416-586-8588
email:     detmar <@t> mshri.on.ca

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Andrea
Hooper
Sent: Friday, April 11, 2008 12:12 PM
To: Michele Wich; Histonet
Subject: Re: [Histonet] Ki-67 for mouse

DAKO clone Tec-3, it's gorgeous on FFPE sections.


>Can someone recommend a good Ki-67 antibody for use on mouse tissue?
>
>Thanks.
>
>
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