[Histonet] Re: Antigenicity lost with decalcification

[Johnson, Teri]

We use Immunocal decalcifier (Decal Corporation) with our mouse tissues and are able to demonstrate GFP with antibody staining just fine. It is a proprietary formic acid mixture, and after fixation is gentle to most antigens.

Hope this helps!

Teri Johnson, HT(ASCP)QIHC
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, MO 64110