[Histonet] Antigenecity lost with decalcification

[Marti Gaudes, Mercè]

Hello.

 

We've done some fluorescent IHC against GFP, but they didn't work.

 

We believe that the GFP antigen(s) are lost during the decalcification process (92% EtOH 70% + 8% Nitric acid for 2 days) but, for example, anti-phospho-Histone3 is working fine.

 

Do you know any decalcification protocol that doesn't interfere with the antigenicity?

 

Thank you!

 

Mercè Martí Gaudes

Cap de Servei

Histology and Bioimaging

Centre de Medicina Regenerativa de Barcelona (CMR[B])
Dr. Aiguader, 88  7ena planta
08003 Barcelona

mmarti <@t> cmrb.eu <mailto:mmarti <@t> cmrbarcelona.org> 

Tel: +34  93 316 03 51

Fax: +34 93 224 10 83