[Histonet] Formalin fixation - how long does it last?

[Rittman, Barry R]

Sonya
Generally you can see some maceration of the tissue if the formalin
bonds start to be broken down.
This generally occurs with large pieces of bone and teeth if
demineralization in EDTA is continued for a lengthy period.
Can then just place in fresh formalin solution for a short time and then
continue with the demineralization.
This is assuming that the original formalin fixation was sufficient.
Barry

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of James S.
Sent: Tuesday, April 08, 2008 7:40 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Formalin fixation - how long does it last?

I have been given some small mouse bones which were fixed in 10%
formalin for 48h but were then transferred to EDTA for decalcification.
They have been left in the EDTA for about 6 weeks. Will they still be
fixed or should I put them back into formalin - or is it too late to do
anything with them? Ideally we want to stain for B cells but could just
do some H&E if we cant get any staining.

Thanks
Sonya


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