[Histonet] F4/80

[Elizabeth M Heimrich]

You also need to be certain of the digestion technique you are using... 
Serotec mouse anti rat F4/80 stains the microglia only if the enzyme 
digestion is used.  ( In my hands anyway!)  Watch your fixation time, as 
well. 
Good luck,
Beth

Geoff McAuliffe wrote:

> Greetings!
>
>    F4/80 does work in mouse brain, staining both "resting" and 
> "activated" microglia". In my hands it is somewhat easier to use than 
> Mac-1 since the rxn.survives fixation better. I would look at your 
> detection system, fresh peroxide, fresh DAB, detection kit mixed 
> properly, etc. Process some liver in parallel as a control.
>
> Geoff
>
> Dr. med. Frauke Neff wrote:
>
>> Dear colleagues,
>> is anybody there who has experiences with a F4/80 antibody? It is 
>> supposed to
>> label "activated" microglia. Unfortunatly I don't get a specific 
>> staining in
>> mice.
>> Does anyone have an idea?
>> Thanks a lot,
>>
>> F. Neff
>>
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>>   
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