[Histonet] F4/80

[Geoff McAuliffe]

Greetings!

    F4/80 does work in mouse brain, staining both "resting" and 
"activated" microglia". In my hands it is somewhat easier to use than 
Mac-1 since the rxn.survives fixation better. I would look at your 
detection system, fresh peroxide, fresh DAB, detection kit mixed 
properly, etc. Process some liver in parallel as a control.

Geoff

Dr. med. Frauke Neff wrote:
> Dear colleagues,
> is anybody there who has experiences with a F4/80 antibody? It is supposed to
> label "activated" microglia. Unfortunatly I don't get a specific staining in
> mice.
> Does anyone have an idea?
> Thanks a lot,
>
> F. Neff
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 
mcauliff <@t> umdnj.edu
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