[Histonet] Goat Collagen I and II HELP

[Pamela Marcum]

Hi All,

I was hoping some one on our lists has successfully gotten collagen 
staining on goat bone.  I have tried five different companies for 
antibodies and none are working on either EDTA decalcified goat bone 
or MMA embedded and de- plasticized goat bone.  These are goat knees 
with the cartilage still attached and some collagen in a defect. have 
also tried three different secondary kits with no luck.  One was an 
Alkaline Phosphatase Kit and two DAB kits with universal 
secondaries.  Please help if you can or tell me it can't be done so I 
can get some rest.

Best Regards,

Pamela A Marcum
Manager, Histology Special Procedures
University of Pennsylvania
School of Veterinary Medicine
R.S. Reynolds Jr.  CORL
New Bolton Center
382 West Street Road
Kennett Square, PA 19348

Phone - 610-925-6278
Fax     - 610-925-8120
E-mail - pmarcum <@t> vet.upenn.edu