[Histonet] Bottomless cup of coffee

[Weems, Joyce]

What kind of hematoxylin? I thought this would be impossible since we no longer use mercury! 
Joyce 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gudrun Lang
Sent: Tuesday, April 01, 2008 12:57 PM
To: 'Marshall Terry Dr,Consultant Histopathologist'
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: AW: [Histonet] Bottomless cup of coffee

If it works, ... it's acceptable. Or have you noticed any problems in staining, that can be caused by this?
And perhaps there are standardizised times and amounts for filling up ;).

I also remember something similiar in our lab before automated staining. And it was a kind of religion, that the haemalaun (Mayers) even would turn better with time in the pot. We always had beautiful HEs. (And now we have
also.)

Gudrun Lang
 
Biomed. Analytikerin
Histolabor
Akh Linz
Krankenhausstr. 9
4020 Linz
+43(0)732/7806-6754
-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Marshall Terry Dr,Consultant Histopathologist
Gesendet: Dienstag, 01. April 2008 17:57
An: histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] Bottomless cup of coffee

 I first came across this concept in Ottawa in 1962 - a cup of coffee that was replenished at no extra charge.
In Oman in 1989, I came across the Indian vegetarian thali that was constantly replenished. (Pearl restaurant in Ruwi - highly recommended).
Now I have come across the bottomless pot of haematoxylin, which has been in use in this lab since I came 8 years ago.
Apparently it is just topped up now and then, and rarely discarded for fresh haematoxylin.
Is this normal/standard/acceptable.
I am shocked to the core!

Terry

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