[Histonet] IHC Forum

[Rene J Buesa]

Hi Patsy:
  
This week there was a discussion in Histonet about IHC on PAP smears and cytospins and using tissue sections as positive controls or not.
   
  Roughly the positions were the following:
   
  1- some accepted using FFPE tissue sections as (+) controls, after HIER
  2- some suggested fixing the smears with NBF and cause the "crossslinkage" to do HIER afterwards
  3- some advocated using ONLY known (+) smears or frozen sections as (+) controls, i.e. non-fixed tissues. The rational being a CAP instruction in which they direct to use controls treated the same as the case.
   
  I think this is an important topic but I also think the "discussion" should be beyond an intelectual argument of each, because that will just expand what has been said this last week.
  I think that with some time ahead some "hard evidence" ought to be presented for discussion, i.e. results obtained using the 3 "approaches" summarized above.
   
  The panel then should view the slides, grade them and get to a determination. The slides should be reviewed/graded "blind", i.e. no one shoudl know the procedure s/he is evaluating.
   
  Give consideration to this proposal.
  René J.

patsy ruegg <pruegg <@t> ihctech.net> wrote:
  

This is a call for IHC questions to be discussed at the NSH S/C in Denver in
OCT.



If you have any burning questions you would like our panel of 9 to address
please email them to me ahead of time.



Thank you,



Patsy Ruegg







Patsy Ruegg, HT(ASCP)QIHC



IHCtech



12635 Montview Blvd. #216



Aurora, CO 80010



720-859-4060



fax 720-859-4110



pruegg <@t> ihctech.net 



www.ihctech.net > 





Patsy Ruegg, HT(ASCP)QIHC

IHCtech

12635 Montview Blvd. #216

Aurora, CO 80010

720-859-4060

fax 720-859-4110

pruegg <@t> ihctech.net

www.ihctech.net 



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