[Histonet] Immuno's on Thin Prep specimens

[Rene J Buesa]

If using thin preps for IHC, you do NOT need to do HIER because they are acetone/air dried fixed, not formalin fixed. The rest of the IHC protocol is the same as for a FFPE tissue section. Your positive control refers to the epitope, so your regular positive control from a tissue section can be used, BUT you negative control has to be a thin prep from the same case simultaneously prepared.
  René J.

AWeiss <@t> shorememorial.org wrote:
  
Is anyone doing IHC's on cytology specimens? If you are can you please
send staining and fixation protocol. Also, what do you use for your
positive and negative controls? Thank you

Andrea J Weiss BST CT (ASCP)
Cytotechnologist
609 653 3577 Ext 4907
aweiss <@t> shorememorial.org
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