[Histonet] Re: Maleic acid vs. Tris in in situ buffer

[Chengkang ZHANG]

Dear All,

I am doing DIG-labeled RNA in situ hybridizations and noticed that some 
protocols use MABT buffer (0.1M maleic acid, 0.15M NaCl, 0.1% Tween-20, 
pH 7.5) to dilute AP-conjugated anti-DIG antibody, while the others use 
TNT buffer (0.1M Tris, 0.15M NaCl, 0.1% Tween-20, pH7.5). The only 
difference between these two buffers is Maleic acid vs. Tris.

I used Tris-based buffer for my Ab solution and it worked for me. I am 
just curious about the difference between maleic acid and Tris. Does 
anyone know if there is any advantage of choosing maleic acid over Tris?

Thanks for your input.

CK.

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Chengkang Zhang Ph.D.
Room 357, MedSurge II
19182 Jamboree Rd,
Department of Pharmacology
University of California, Irvine
Irvine, CA 92697-4625
Email:  chengkaz <@t> uci.edu
Tel:    (949)-824-1902 (lab)
Fax:    (949)-824-4855
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