[Histonet] whole mount

[Valeria Berno]

Hi,
I was just wondering if it is common to use Sudan Black or other
autofluorescence quenching method on whole mounting.It is the first time I
am approaching whole mount immuno!!.

Is there any special suggestion as my antigene has a very low expression??

thanks in advance

Valeria 
 
Berno Valeria, PhD
 

EMBL Monterotondo Outstation

via Ramarini 32

00015 Monterotondo Scalo (RM)

Italy

Tel:  +39 06 90091 287

Fax: +39 06 90091 272

HYPERLINK "mailto:valeria.berno <@t> embl.it"valeria.berno <@t> embl.it

 

No virus found in this outgoing message.
Checked by AVG Free Edition. 
Version: 7.5.488 / Virus Database: 269.13.30/1025 - Release Date: 23/09/2007
13.53