[Histonet] Thin slice embedding

[Shengwen Zhang]

Hi All,

I'm trying to further cut my organotypically cultured brain slices (300 micron before culture, ~100 micron after culture).  I've tried OTC compound and sucrose solutions for embedding, both didn't work well (the slices wrinkle at the tissue-medium boundary).  Now I want to try paraffin.  I want to keep 3-4 slices from the same brain flat and on the same plane, so I can process them together (otherwise, I would have too much work to do).  I created a flat area with paraffin, after it solidified, I laid the slices, and pour another layer of paraffin.  But the two layers don't stick together well enough to stand the forces during cutting.  How do you embed thin slices with paraffin?

Thank you!

Shengwen