[Histonet] PBS/MgCl2

Gudrun Lang gu.lang <@t> gmx.at
Thu Sep 20 11:40:30 CDT 2007

Hi FISHers,

I am quite new in FISH-techniques. I have a protocol for
imprint-preparation, where PBS/MgCl2 follows the pepsin digestionstep. Can
anybody tell me, why I have to use PBS with MgCl2 to stop digestion? Isn't
it the same, to use 1xPBS alone? Further there are different recipes for the
solution. (5% 1M-MgCl2 in 1x PBS; 1000ml 1xPBS with 1,015 g MgCl2) That
means one is 10fold richer on MgCls than the other.

What pH is the right one here?


Many questions, hot brain, help appreciated..



More information about the Histonet mailing list