[Histonet] paraffin processing problem

Carla M Conway cmconway <@t> usgs.gov
Wed Sep 19 10:54:37 CDT 2007


I have an LX120 tissue processor. The paraffin infiltration time is
programmed for  three 1hour stations. Sometime last night a problem
occurred during the paraffin cycle (unfortunately, we do not have a
printout or record of when it happened).  This morning the Fill Failure
alarm was sounding and tissues were not soaking in paraffin as they should
have been. There was only  paraffin residue in the processing chamber, so I
know that the tissues were exposed to paraffin but don't know for how long.
What should I do? I do not want to overprocess them in paraffin, but don't
want them underinfiltrated either. The tissues are staghorn sculpin hearts
and livers.

Thanks so much for your help,

Carla Conway

Western Fisheries Research Center
6505 NE 65th St.
Seattle, WA 98115
cmconway <@t> usgs.gov

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