[Histonet] Background on FFPE mouse tissue

[Gale, Nadia]

Hello Histonetters,
 
I am relatively new to the mouse world of IHC having come from a
clinical background into research and have what might be a simple
problem... I am experiencing heavy background with the majority of mouse
tissues I am staining.  I am using the Ventana Discovery system with
their universal DAB kit and "titrate" 100uL of antibody diluent onto my
negative control slides.  I've tried a biotin blocking kit as well (even
though it doesn't look like biotin, thought I'd give it a try) and
observed no decrease in the amount of background staining.
 
Any ideas for me?
 
 
Thanks in advance!
Nadia
 
Nadia Gale
Lead Histotechnologist
Centre for Translational and Applied Genomics (CTAG)
3427-600 West 10th Avenue
Vancouver, BC V5Z 4E6
tel 604-877-6000 ext 2426
fax 604-877-6089
ngale <@t> bccancer.bc.ca