[Histonet] air bubbles seen after mounting with entellan

[Moran Elishmereni]

Dear Histonetters,

I am getting a lot of air bubbles after coversliping my slides with entellan
(following neo-clear, a xylene substitute). I made sure that the slides
are wet with the neo-clear, and then applied the mounting media. Why is this
happening? I have to add that I used cold entellan once and this didn't
happen, but when the entellan was in room temp (and therefore less viscous),
it did. Does viscosity have anything to do with it? I'd appreciate any piece
of advice on how to avoid these air bubbles!

Many thanks,
Moran Elishmereni

Department of Pharmacology and Experimental Therapeutics
School of Pharmacy, Faculty of Medicine
The Hebrew University of Jerusalem
POB 12065
Jerusalem 91120, ISRAEL
Tel: 972-2-675-8746
Fax: 972-2-675-8144
Email: moran.elish <@t> gmail.com