[Histonet] negative controls

[Rene J Buesa]

One negative per block tested!
  You need to know if any reaction in any block (section) is of positive or a negative (background) nature.
  Another thing would be the positive control. You need only one positive control, because in that case you are testing the Ab reactivity.
  René J.

godsgalnow <@t> aol.com wrote:
  How many non-CAP labs out there are doing negative controls on every block that you do an antibody on?

If you have a case that goes A-J and they are all 1 block each and you do an AE1/AE3 on blocks B,C,F,& J, will you do a negative on every block or just one for the entire case?

This has been an ongoing debate with us.? We typincally only do 1 negative control for the case and given the above example, it might be on block A.? We ususally cut an extra on every block we cut and when the IHC is requested we just go and pull it and for the negative, we just grab whatever slide is left to run the negative on.? What is everyone else doing?? 

Roxanne
________________________________________________________________________
Email and AIM finally together. You've gotta check out free AOL Mail! - http://mail.aol.com
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




       
---------------------------------
Take the Internet to Go: Yahoo!Go puts the Internet in your pocket: mail, news, photos & more.