[Histonet] Cutting rat brains

[Gayle Callis]

You can purchase a brain matrix device, from many places. Try Charles 
Scouten's website, Google  his website with keey word myneurolab.  These 
devices allow you to section rat brains by placing the brain in a form, and 
then making precise slices, then you can proceed with how you want to 
orient, etc.

You need to make sure the brains are kept flat during snap freezing after 
the slices are made.  There is a way to do this using a liquid 
nitrogen/petri dish setup.  If you wish I will send the mini powerpoint on 
how to do this.

At 07:30 PM 9/13/2007, you wrote:
>I want to cut a frozen rat brain at around 1-3 mm intervals to form blocks.
>I wish to be able to return to each block at a later date to take further
>sections if necessary.  Can anyone suggest a suitable method or piece of
>equipment that would aid this task?  Thanks
>
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610