[Histonet] Spur Resin
Malinowski, Joanne O,
Joanne.Malinowski <@t> crl.com
Wed Sep 12 16:13:16 CDT 2007
Hello Plastic People,
Could anyone share their recipe for using spur resin to produce plastic sections? Thank you all in advance for offering any help.
Best regards,
Joanne
Joanne Malinowski,HT ASCP
Plastics Lab Manager, Medical Devices Division
Charles River Laboratories
Pathology Associates
15 Worman's Mill Court, Suite I
Frederick, Maryland 21701
Phone 301-624-2034
Fax 301-663-8994
Email: joanne.malinowski <@t> us.crl.com
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Wednesday, September 12, 2007 12:46 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 46, Issue 18
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Today's Topics:
1. Re: Worlds Largest Paraffin Dispenser (Joe Nocito)
2. RE: Lymph node fixative/enhance solutions (Mike Pence)
3. Dissect Aid (Orr, Rebecca)
4. Re: Worlds Largest Paraffin Dispenser (Robyn Vazquez)
5. Histology Consumables Product Survey ($50 Honorarium
opportunity) (Adam Goldstein)
6. Re: Lymph node fixative/enhance solutions (Robert Richmond)
7. Re: Worlds Largest Paraffin Dispenser (Joe Nocito)
8. Looking for a specific type of slide tray..... (Mindy Johnson)
9. RE: Worlds Largest Paraffin Dispenser (Bernice Frederick)
10. Opinion on Microm automated microtomes? (Kriederman, Ben)
11. Re: Worlds Largest Paraffin Dispenser (Larry Woody)
12. RE: Looking for a specific type of slide tray..... (Horn, Hazel V)
13. RE: Opinion on Microm automated microtomes? (Ford, Judi)
14. RE: Looking for a specific type of slide tray..... (Mindy Johnson)
15. Special Stainers (Rebecca Johnson)
16. Special Stainers (Rebecca Johnson)
17. Histnet Special Stainers (Rebecca Johnson)
18. Re: Opinion on Microm automated microtomes? (ribonukle)
19. Re: Opinion on Microm automated microtomes? (Anthony Reilly)
20. co-localisation within a cell using DAB and Vulcan Fast Red
(Cathy Malcontenti-Wilson)
21. AO 860 sliding microtome (Caroline Bass)
22. QC labeling (Marcia Funk)
23. Re: AO 860 sliding microtome (Rene J Buesa)
24. Re: QC labeling (Rene J Buesa)
25. Re: AO 860 sliding microtome
(mari.ann.mailhiot <@t> leica-microsystems.com)
----------------------------------------------------------------------
Message: 1
Date: Tue, 11 Sep 2007 12:02:57 -0500
From: "Joe Nocito" <jnocito <@t> satx.rr.com>
Subject: Re: [Histonet] Worlds Largest Paraffin Dispenser
To: "Douglas D Deltour" <doug <@t> ppspath.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <006201c7f495$9c293840$0202a8c0 <@t> yourxhtr8hvc4p>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original
I know TBS and ThermoFisher have 5 gallon pots. How big do you need? I'm
getting visions of hot paraffin in a hot tub.
Any vendors want to try that for Denver? I mean, it will be the end of
October.
Joe
----- Original Message -----
From: "Douglas D Deltour" <doug <@t> ppspath.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, September 11, 2007 11:49 AM
Subject: [Histonet] Worlds Largest Paraffin Dispenser
>I am looking for a paraffin dispenser that holds a LARGE amount of
>paraffin.
> My requests for a hot tub that I can fill with paraffin have been denied
> for
> some reason. Anyone know of a manufacturer of these large dispensers?
>
>
>
> Douglas D. Deltour HT(ASCP)
>
> Histology Manager
>
> Professional Pathology Services, PC
>
> One Science Court
>
> Suite 200
>
> Columbia, SC 29203
>
> Office (803)252-1913
>
> Fax (803)254-3262
>
> Doug <@t> ppspath.com
>
> *****************************************************
>
> PROFESSIONAL PATHOLOGY SERVICES, PC
> NOTICE OF CONFIDENTIALITY
> This message is intended only for the use of the individual or entity to
> which it is addressed and may contain information that is privileged,
> confidential and exempt from disclosure under applicable law. If the
> reader
> of this message is not the intended recipient, you are hereby notified
> that
> any dissemination, distribution, or copying of this communication is
> strictly prohibited by law. If you have received this communication in
> error, please notify me immediately.
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 2
Date: Tue, 11 Sep 2007 12:06:27 -0500
From: "Mike Pence" <mpence <@t> grhs.net>
Subject: RE: [Histonet] Lymph node fixative/enhance solutions
To: "Cindy Robinson" <robinsoc <@t> mercyhealth.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C714 <@t> IS-E2K3.grhs.net>
Content-Type: text/plain; charset="us-ascii"
We used straight acetone and the fat turns translucent and the nodes
turn white within 10 minutes. Easy to get, cheap and no mixing or
purchasing reagents.
Mike
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cindy
Robinson
Sent: Tuesday, September 11, 2007 11:24 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Lymph node fixative/enhance solutions
Our pathologists would like us to start using a solution to help with
the dissection and ID of lymph nodes at gross. I have done some
searching thru the archives and found some recipes but would like to
find a commercial source. We do use Pen-Fix but it doesn't dissolve the
fat like the paths would like. Has anyone got a source or recipe for a
fat dissolving solution that they would like to share?
Thanks.
Cindi Robinson, HT (ASCP)
Dunes Medical Laboratories
Dakota Dunes SD 57049
_______________________________________________
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Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 3
Date: Tue, 11 Sep 2007 12:31:49 -0500
From: "Orr, Rebecca" <ROrr <@t> enh.org>
Subject: [Histonet] Dissect Aid
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<DB61DED572F83344AE6894373372486B9771C9 <@t> EXCHANGE03.enhnet.org>
Content-Type: text/plain; charset="us-ascii"
We use Dissect Aid in our gross room for the application you've
referenced.
Thanks for sharing the Home Brew though!
We get it from Decal Corp and it works very well
It's got Acetic Acid and Formalin (40% I believe)
And prolly some other stabilizer.
Becky Orr CLA,HT(ASCP)QIHC
Anatomic Pathology
Evanston Northwestern Healthcare
847-570-2771
------------------------------
Message: 4
Date: Tue, 11 Sep 2007 10:38:36 -0700
From: "Robyn Vazquez" <vazquezr <@t> ohsu.edu>
Subject: Re: [Histonet] Worlds Largest Paraffin Dispenser
To: histonet <@t> lists.utsouthwestern.edu, doug <@t> ppspath.com
Message-ID: <s6e67035.064 <@t> OHSU.EDU>
Content-Type: text/plain; charset=us-ascii
Hacker carries them, around $1000.00.
Robyn
>>> "Douglas D Deltour" <doug <@t> ppspath.com> 9/11/2007 9:49 AM >>>
I am looking for a paraffin dispenser that holds a LARGE amount of
paraffin.
My requests for a hot tub that I can fill with paraffin have been
denied for
some reason. Anyone know of a manufacturer of these large dispensers?
Douglas D. Deltour HT(ASCP)
Histology Manager
Professional Pathology Services, PC
One Science Court
Suite 200
Columbia, SC 29203
Office (803)252-1913
Fax (803)254-3262
Doug <@t> ppspath.com
*****************************************************
PROFESSIONAL PATHOLOGY SERVICES, PC
NOTICE OF CONFIDENTIALITY
This message is intended only for the use of the individual or entity
to
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of this message is not the intended recipient, you are hereby notified
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------------------------------
Message: 5
Date: Tue, 11 Sep 2007 13:45:00 -0400
From: "Adam Goldstein" <agoldstein <@t> KaiserAssociates.com>
Subject: [Histonet] Histology Consumables Product Survey ($50
Honorarium opportunity)
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<D08FAE758C61D740A1B581D2A60BAA71031A287B <@t> KADC02.kaiserassociates.com>
Content-Type: text/plain; charset="us-ascii"
Dear Histonetters,
Our firm, Kaiser Associates, is conducting research on the products and
processes for the histologic technique of primary (H&E) staining and
would like your input. By participating in this survey, you will be
influencing potential upcoming changes to the market for primary (H&E)
staining products. The completion of this survey should only take 20-30
minutes, and in return for your participation you will receive an
honorarium of US $50 in the form of an American Express gift card. If
you would like to participate in this survey please reply to
AGoldstein <@t> KaiserAssociates.com with:
1. Your job title;
2. Your department; and
3. Your company/hospital name and location
Upon receipt of the above information, we will send you the web link to
the survey. Please note that we are primarily interested in respondents
from the US or Europe. Thanks!
Sincerely,
Kaiser Associates, Inc. Research Team
About Kaiser Associates, Inc:
Kaiser Associates is a research-based international consulting firm
dedicated to helping leading global corporations develop effective
strategies to drive continued operating performance. For more
information please go to www.KaiserAssociates.com.
------------------------------
Message: 6
Date: Tue, 11 Sep 2007 13:52:33 -0400
From: "Robert Richmond" <RSRICHMOND <@t> aol.com>
Subject: [Histonet] Re: Lymph node fixative/enhance solutions
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<abea52a60709111052m13685e9eu8d15d9aa301447f <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Cindi Robinson, HT (ASCP) in South Dakota asks:
>>Our pathologists would like us to start using a solution to help
with the dissection and identification of lymph nodes at gross. - We
do use Pen-Fix but it doesn't dissolve the fat like the paths would
like.<<
And Dana Dittus at Polysciences notes:
>>Polysciences,Inc manufactures Hartmanns Fixative which turns lymph
nodes white. For more info see www.polysciences.com <<
It's easy and cheap to prepare Davidson's fixative (same as Hartmann's
fixative) - mix 3 parts water, 3 parts reagent alcohol, 2 parts strong
(37%) formalin, not the buffered kind, and 1 part glacial acetic acid.
Color it with eosin if you want to.
When Dr. William Hartmann (later the chair of the American Board of
Pathology) was at Johns Hopkins in the early 1960's, Dr. Victor
McCusick (the medical geneticist, later chair of medicine at Hopkins)
asked him to introduce the fixative for Barr bodies (sex chromatin
bodies) that Moore and Barr had specified in their original articles
on Barr bodies around 1954. Moore and Barr specified "modified
Davidson's fixative", which Bill Hartmann introduced. It came into
rather widespread use at Hopkins, and Hartmann's name stuck to it. I
think the name should not be used, because of possible disastrous
confusion with Hartmann's solution, lactated Ringer's solution,
introduced into pediatric practice in the 1930's by Dr. Alexis
Hartmann Sr. at Washington University in St.Louis. Dr. Alexis Hartmann
was one of my clinical teachers, right before he retired around 1964
(sorry about all this name dropping).
John Kiernan and I discussed Davidson's fixative in this forum several
years ago. He noted that it is not really a rational formula. He hoped
to have the time to research the fixative in Davidson's papers, since
it seems never to have been published.
Getting back to the actual subject, I've used Dissect Aid (Decal
Corporation) with good results - got a bottle of it on the shelf right
now, in fact. In my brief experience with Penn-Fix, it did not
disclose lymph nodes well.
Let me support your pathologists very strongly in this endeavor. Just
one positive lymph node upstages a colon cancer and makes chemotherapy
mandatory. And in colon cancer, those postive lymph nodes can be the
size of pinheads.
Bob Richmond
Samurai Pathologist
Knoxville TN
------------------------------
Message: 7
Date: Tue, 11 Sep 2007 12:02:57 -0500
From: "Joe Nocito" <jnocito <@t> satx.rr.com>
Subject: Re: [Histonet] Worlds Largest Paraffin Dispenser
To: "Douglas D Deltour" <doug <@t> ppspath.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000001c7f4a9$fcd58310$0202a8c0 <@t> yourxhtr8hvc4p>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original
I know TBS and ThermoFisher have 5 gallon pots. How big do you need? I'm
getting visions of hot paraffin in a hot tub.
Any vendors want to try that for Denver? I mean, it will be the end of
October.
Joe
----- Original Message -----
From: "Douglas D Deltour" <doug <@t> ppspath.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, September 11, 2007 11:49 AM
Subject: [Histonet] Worlds Largest Paraffin Dispenser
>I am looking for a paraffin dispenser that holds a LARGE amount of
>paraffin.
> My requests for a hot tub that I can fill with paraffin have been denied
> for
> some reason. Anyone know of a manufacturer of these large dispensers?
>
>
>
> Douglas D. Deltour HT(ASCP)
>
> Histology Manager
>
> Professional Pathology Services, PC
>
> One Science Court
>
> Suite 200
>
> Columbia, SC 29203
>
> Office (803)252-1913
>
> Fax (803)254-3262
>
> Doug <@t> ppspath.com
>
> *****************************************************
>
> PROFESSIONAL PATHOLOGY SERVICES, PC
> NOTICE OF CONFIDENTIALITY
> This message is intended only for the use of the individual or entity to
> which it is addressed and may contain information that is privileged,
> confidential and exempt from disclosure under applicable law. If the
> reader
> of this message is not the intended recipient, you are hereby notified
> that
> any dissemination, distribution, or copying of this communication is
> strictly prohibited by law. If you have received this communication in
> error, please notify me immediately.
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 8
Date: Tue, 11 Sep 2007 12:58:31 -0700
From: "Mindy Johnson" <m5johnso <@t> meded.ucsd.edu>
Subject: [Histonet] Looking for a specific type of slide tray.....
To: "'HistoNet'" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <007301c7f4ae$212c80c0$63858240$@ucsd.edu>
Content-Type: text/plain; charset="us-ascii"
Hi All-
We are looking for some trays that hold slides. We currently have about 3
and I need more. They look old school and I have no clue the technical term
for them. But, they hold about 50 slides (5 rows hold about 10 each), are
flat on one side and the other is divided into the 5 rows, the material is
made of that stuff that resembles a wood like fiberboard. They are
extremely handy for our lab and good for after cutting I put in the
incubator to make sure they are totally dry before staining. Anyone have
any idea where I can find something similar to this?
Thank you so much for your help!
Mindy
------------------------------
Message: 9
Date: Tue, 11 Sep 2007 15:00:52 -0500
From: "Bernice Frederick" <b-frederick <@t> northwestern.edu>
Subject: RE: [Histonet] Worlds Largest Paraffin Dispenser
To: "'Joe Nocito'" <jnocito <@t> satx.rr.com>, "'Douglas D Deltour'"
<doug <@t> ppspath.com>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <001a01c7f4ae$77f76fa0$d00f7ca5 <@t> lurie.northwestern.edu>
Content-Type: text/plain; charset="us-ascii"
One BIG candle for someones birthday!!!!!!!!!
Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Joe Nocito
Sent: Tuesday, September 11, 2007 12:03 PM
To: Douglas D Deltour; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Worlds Largest Paraffin Dispenser
I know TBS and ThermoFisher have 5 gallon pots. How big do you need? I'm
getting visions of hot paraffin in a hot tub.
Any vendors want to try that for Denver? I mean, it will be the end of
October.
Joe
----- Original Message -----
From: "Douglas D Deltour" <doug <@t> ppspath.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, September 11, 2007 11:49 AM
Subject: [Histonet] Worlds Largest Paraffin Dispenser
>I am looking for a paraffin dispenser that holds a LARGE amount of
>paraffin.
> My requests for a hot tub that I can fill with paraffin have been denied
> for
> some reason. Anyone know of a manufacturer of these large dispensers?
>
>
>
> Douglas D. Deltour HT(ASCP)
>
> Histology Manager
>
> Professional Pathology Services, PC
>
> One Science Court
>
> Suite 200
>
> Columbia, SC 29203
>
> Office (803)252-1913
>
> Fax (803)254-3262
>
> Doug <@t> ppspath.com
>
> *****************************************************
>
> PROFESSIONAL PATHOLOGY SERVICES, PC
> NOTICE OF CONFIDENTIALITY
> This message is intended only for the use of the individual or entity to
> which it is addressed and may contain information that is privileged,
> confidential and exempt from disclosure under applicable law. If the
> reader
> of this message is not the intended recipient, you are hereby notified
> that
> any dissemination, distribution, or copying of this communication is
> strictly prohibited by law. If you have received this communication in
> error, please notify me immediately.
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 10
Date: Tue, 11 Sep 2007 12:05:43 -0800
From: "Kriederman, Ben" <Benjamin.Kriederman <@t> providence.org>
Subject: [Histonet] Opinion on Microm automated microtomes?
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<D389085B3E175D408C11FFD1AB3FFD7E05672353 <@t> wn0152.ak.providence.org>
Content-Type: text/plain; charset="iso-8859-1"
Does anyone have an opinion on the microm hm355s automated microtome? When it comes to cutting hard tissues we are getting thick and thin, any suggestions?
Thick and Thin in Alaska
DISCLAIMER:
This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message.
------------------------------
Message: 11
Date: Tue, 11 Sep 2007 13:10:54 -0700 (PDT)
From: Larry Woody <slappycraw <@t> yahoo.com>
Subject: Re: [Histonet] Worlds Largest Paraffin Dispenser
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <308814.42020.qm <@t> web53605.mail.re2.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
I've heard of people getting into their work before but embedding yourself?
---------------------------------
Be a better Heartthrob. Get better relationship answers from someone who knows.
Yahoo! Answers - Check it out.
------------------------------
Message: 12
Date: Tue, 11 Sep 2007 15:34:29 -0500
From: "Horn, Hazel V" <HornHV <@t> archildrens.org>
Subject: RE: [Histonet] Looking for a specific type of slide tray.....
To: "Mindy Johnson" <m5johnso <@t> meded.ucsd.edu>, "HistoNet"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<9AE8AA9E1F644B4AA6C155FB6FD51C630ACB7335 <@t> EMAIL.archildrens.org>
Content-Type: text/plain; charset=us-ascii
I know I ordered some of these trays before and the company was called
brain research labs? But I looked on the internet and cannot find
anything. The flats were trimmed in wood with a white background.
Each row held 10 slides and there are 5 rows on the board. Maybe
someone else can help you.
Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Histology
Arkansas Children's Hospital
800 Marshall Slot 820
Little Rock, AR 72202
phone 501.364.4240
fax 501.364.3912
visit us on the web at: www.archildrens.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mindy
Johnson
Sent: Tuesday, September 11, 2007 2:59 PM
To: 'HistoNet'
Subject: [Histonet] Looking for a specific type of slide tray.....
Hi All-
We are looking for some trays that hold slides. We currently have about
3
and I need more. They look old school and I have no clue the technical
term
for them. But, they hold about 50 slides (5 rows hold about 10 each),
are
flat on one side and the other is divided into the 5 rows, the material
is
made of that stuff that resembles a wood like fiberboard. They are
extremely handy for our lab and good for after cutting I put in the
incubator to make sure they are totally dry before staining. Anyone
have
any idea where I can find something similar to this?
Thank you so much for your help!
Mindy
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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------------------------------
Message: 13
Date: Tue, 11 Sep 2007 13:38:56 -0700
From: "Ford, Judi" <judi.ford <@t> roche.com>
Subject: RE: [Histonet] Opinion on Microm automated microtomes?
To: "Kriederman, Ben" <Benjamin.Kriederman <@t> providence.org>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<DF7DFE066B39BB429DFBCCC82FFB7AB905D4C38C <@t> rplmsem1.nala.roche.com>
Content-Type: text/plain; charset="us-ascii"
I've been working with that model for quite sometime now and once in
awhile I get thick and thin sections. Usually it happens when something
is loose or I'm cutting in the middle of the blade. I tend to cut on
either end of the knife blade, avoiding the middle of the blade holder
when I can. Check the knife angle (9 or 10). Also, try checking the
block to see that it is properly seated in the chuck and tight.
Hope this helps. Good luck.
Judi Ford
Palo Alto, CA
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Kriederman, Ben
Sent: Tuesday, September 11, 2007 1:06 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Opinion on Microm automated microtomes?
Does anyone have an opinion on the microm hm355s automated microtome?
When it comes to cutting hard tissues we are getting thick and thin, any
suggestions?
Thick and Thin in Alaska
DISCLAIMER:
This message is intended for the sole use of the addressee, and may
contain information that is privileged, confidential and exempt from
disclosure under applicable law. If you are not the addressee you are
hereby notified that you may not use, copy, disclose, or distribute to
anyone the message or any information contained in the message. If you
have received this message in error, please immediately advise the
sender by reply email and delete this message.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 14
Date: Tue, 11 Sep 2007 14:15:42 -0700
From: "Mindy Johnson" <m5johnso <@t> meded.ucsd.edu>
Subject: RE: [Histonet] Looking for a specific type of slide tray.....
To: "'Horn, Hazel V'" <HornHV <@t> archildrens.org>, "'HistoNet'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <007e01c7f4b8$e9c5c280$bd514780$@ucsd.edu>
Content-Type: text/plain; charset="iso-8859-1"
Yes it is Brain Research Labs! They are exactly what I was looking for!
THANK YOU SOOOO MUCH!!
Mindy A Johnson
SRA II
UCSD - School of Medicine
Medical Teaching Labs
-----Original Message-----
From: Horn, Hazel V [mailto:HornHV <@t> archildrens.org]
Sent: Tuesday, September 11, 2007 1:34 PM
To: Mindy Johnson; HistoNet
Subject: RE: [Histonet] Looking for a specific type of slide tray.....
I know I ordered some of these trays before and the company was called
brain research labs? But I looked on the internet and cannot find
anything. The flats were trimmed in wood with a white background.
Each row held 10 slides and there are 5 rows on the board. Maybe
someone else can help you.
Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Histology
Arkansas Children's Hospital
800 Marshall Slot 820
Little Rock, AR 72202
phone 501.364.4240
fax 501.364.3912
visit us on the web at: www.archildrens.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mindy
Johnson
Sent: Tuesday, September 11, 2007 2:59 PM
To: 'HistoNet'
Subject: [Histonet] Looking for a specific type of slide tray.....
Hi All-
We are looking for some trays that hold slides. We currently have about
3
and I need more. They look old school and I have no clue the technical
term
for them. But, they hold about 50 slides (5 rows hold about 10 each),
are
flat on one side and the other is divided into the 5 rows, the material
is
made of that stuff that resembles a wood like fiberboard. They are
extremely handy for our lab and good for after cutting I put in the
incubator to make sure they are totally dry before staining. Anyone
have
any idea where I can find something similar to this?
Thank you so much for your help!
Mindy
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Message: 15
Date: Tue, 11 Sep 2007 18:32:37 -0500
From: "Rebecca Johnson" <raj <@t> bluemarble.net>
Subject: [Histonet] Special Stainers
To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <011a01c7f4cc$09fefcc0$7b48f9d8 <@t> CHURCH>
Content-Type: text/plain; charset="iso-8859-1"
What special stainers is everyone using? I now have a Ventana Nexus. Bought in 1999. I know this is old, but it has been maintained well. We have had problems with it several times over the years. Mostly with sliver stains or uneven staining and no staining. I would like to know what others are using and your experiences are. Thanks for all you help.
raj
------------------------------
Message: 16
Date: Tue, 11 Sep 2007 18:39:37 -0500
From: "Rebecca Johnson" <raj <@t> bluemarble.net>
Subject: [Histonet] Special Stainers
To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <012801c7f4cd$045aa1b0$7b48f9d8 <@t> CHURCH>
Content-Type: text/plain; charset="iso-8859-1"
Need advice on special stainers everyone is using. We now have Ventana Nexus special stainer.Bought in 1999. I know this is old but it has been maintained well. We have always had a service contract with Ventana. Have had problems over the years with the silver stains, no staining and uneven staining. Now they want to tell me that this is normal with any company. For the amount of money these stainers cost that is just little more than I am willing to believe.
Thanks for all you help.
raj
------------------------------
Message: 17
Date: Tue, 11 Sep 2007 19:00:33 -0500
From: "Rebecca Johnson" <raj <@t> bluemarble.net>
Subject: [Histonet] Histnet Special Stainers
To: "histonet" <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <017101c7f4cf$f0967bb0$7b48f9d8 <@t> CHURCH>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original
Need advice on what special stainers everyone is using. The good and bad.
We are now using Ventana Nexus special stainer. Bought in 1999. Not real
happy with what I am hearing about all stainers. I was told today by a
manager that all stainers have problems a couple times a year. We have had
uneven staining, no staining and problems with the silver stain. For what
they cost and the cost of the maintenance contracts, this is hard for me to
believe. What is your experience with the stainers.
Thanks
raj
------------------------------
Message: 18
Date: Tue, 11 Sep 2007 20:33:58 -0300
From: "ribonukle" <Ribonukle <@t> yahoo.com.ar>
Subject: Re: [Histonet] Opinion on Microm automated microtomes?
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <01e801c7f4cc$3b8adb10$590215ac <@t> Pentium4>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original
I have a similar model and also has that problem, thick and thin with all
very good adjusted.they have already become unstuck and broken several
things
This microm Microtome have low quality
Hope this helps.
Ribo
----- Original Message -----
From: "Ford, Judi" <judi.ford <@t> roche.com>
To: "Kriederman, Ben" <Benjamin.Kriederman <@t> providence.org>;
<histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, September 11, 2007 5:38 PM
Subject: RE: [Histonet] Opinion on Microm automated microtomes?
I've been working with that model for quite sometime now and once in
awhile I get thick and thin sections. Usually it happens when something
is loose or I'm cutting in the middle of the blade. I tend to cut on
either end of the knife blade, avoiding the middle of the blade holder
when I can. Check the knife angle (9 or 10). Also, try checking the
block to see that it is properly seated in the chuck and tight.
Hope this helps. Good luck.
Judi Ford
Palo Alto, CA
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Kriederman, Ben
Sent: Tuesday, September 11, 2007 1:06 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Opinion on Microm automated microtomes?
Does anyone have an opinion on the microm hm355s automated microtome?
When it comes to cutting hard tissues we are getting thick and thin, any
suggestions?
Thick and Thin in Alaska
DISCLAIMER:
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------------------------------
Message: 19
Date: Wed, 12 Sep 2007 10:01:05 +1000
From: "Anthony Reilly" <Tony_Reilly <@t> health.qld.gov.au>
Subject: Re: [Histonet] Opinion on Microm automated microtomes?
To: <histonet <@t> lists.utsouthwestern.edu>,<Ribonukle <@t> yahoo.com.ar>
Message-ID: <s6e7b8ed.087 <@t> health.qld.gov.au>
Content-Type: text/plain; charset="us-ascii"
I agree. I have 2 old Microms purchased in the early nineties which
were very good on purchase and are still going strong however we have
another that was purchased about 2 years ago and there is a marked
decrease in the sectioning ability. Even the physical appearance of the
instument is notably inferior. Some blocks will cut thick and thin for
no apparent reason. They do not even have to be hard tissue.
regards
Tony Reilly
Chief Scientist
Anatomical Pathology
QHPS-Prince Charles Hospital
Rode Rd Chermside Q 4032
Australia
Ph: 07 3139 4543
Fax: 07 3193 4546
tony_reilly <@t> health.qld.gov.au
>>> "ribonukle" <Ribonukle <@t> yahoo.com.ar> 09/12/07 9:33 am >>>
I have a similar model and also has that problem, thick and thin with
all
very good adjusted.they have already become unstuck and broken several
things
This microm Microtome have low quality
Hope this helps.
Ribo
----- Original Message -----
From: "Ford, Judi" <judi.ford <@t> roche.com>
To: "Kriederman, Ben" <Benjamin.Kriederman <@t> providence.org>;
<histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, September 11, 2007 5:38 PM
Subject: RE: [Histonet] Opinion on Microm automated microtomes?
I've been working with that model for quite sometime now and once in
awhile I get thick and thin sections. Usually it happens when
something
is loose or I'm cutting in the middle of the blade. I tend to cut on
either end of the knife blade, avoiding the middle of the blade holder
when I can. Check the knife angle (9 or 10). Also, try checking the
block to see that it is properly seated in the chuck and tight.
Hope this helps. Good luck.
Judi Ford
Palo Alto, CA
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Kriederman, Ben
Sent: Tuesday, September 11, 2007 1:06 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Opinion on Microm automated microtomes?
Does anyone have an opinion on the microm hm355s automated microtome?
When it comes to cutting hard tissues we are getting thick and thin,
any
suggestions?
Thick and Thin in Alaska
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Message: 20
Date: Wed, 12 Sep 2007 15:28:32 +1000
From: Cathy Malcontenti-Wilson <cmalc <@t> unimelb.edu.au>
Subject: [Histonet] co-localisation within a cell using DAB and Vulcan
Fast Red
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<6.2.1.2.2.20070912152507.0421f230 <@t> mail.staff.unimelb.edu.au>
Content-Type: text/plain; charset="us-ascii"; format=flowed
Dear Histonetters,
Does anyone have experience with co-localisation of the chromogens DAB and
Vulcan Fast Red in an immunohistochemical stain? I have just done a double
stain and I would like to know what I should I expect if the two antigens I
have stained are co-localised in the one cell? Would it to look red or
orange or what??
Much appreciated,
Thanks
Cathy Malcontenti-Wilson
------------------------------
Message: 21
Date: Wed, 12 Sep 2007 10:58:54 -0400
From: Caroline Bass <cbass <@t> wfubmc.edu>
Subject: [Histonet] AO 860 sliding microtome
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <C30D76EE.5B23%cbass <@t> wfubmc.edu>
Content-Type: text/plain; charset="US-ASCII"
Hi guys,
So I've inherited an American Optical 860 sliding microtome. I consider
this quite a find since it is my all time favorite for slicing fixed brains.
However, it has been packed away for quite a while and seems to be a bit
gummed up and is in parts. Does anyone have suggestions on how to get this
back into working order. I don't suppose there is a manual floating around?
Any general suggestions for degreasing to remove the stick points. How
about a location for spare parts, particularly dry ice stages and maybe a
disposable blade holder?
Any and all suggestions are appreciated.
Thanks,
Caroline Bass
------------------------------
Message: 22
Date: Wed, 12 Sep 2007 11:29:57 -0400
From: "Marcia Funk" <FUNKM <@t> mercyhealth.com>
Subject: [Histonet] QC labeling
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <46E7BFA5.9B87.00AC.0 <@t> mercyhealth.com>
Content-Type: text/plain; charset=us-ascii
Histo Friends,
I have a few questions I would like to see how labs are following through with labeling errors?
Case number placed on slide during sectioning. Slide is then mislabeled handed off to pathologist.
Pathologist discovers the error. Slide is labeled incorrectly.
Wrong label placed on slide during labeling. Pathologist discovers mislabeld slide
Errors are place on QC log at the end of day. These are errors that are caught in the department
by the tech or pathologist. Do most labs keep a log with errors by tech and how many errors
impact there eval ?
I would appreciate your imput.
Mrf
------------------------------
Message: 23
Date: Wed, 12 Sep 2007 08:31:03 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] AO 860 sliding microtome
To: Caroline Bass <cbass <@t> wfubmc.edu>,
histonet <@t> lists.utsouthwestern.edu
Message-ID: <753832.79448.qm <@t> web61225.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Caroline:
I learned to cut in one of those (in 1952) and I still consider this model of horizontal microtome the finest ever built, specially with its clever vertical automatic feeding mechanism.
IF you have mechanical dextereity, it is not difficult to put it back in working conditoins. As you can see, almost every part is screwed and even the feeding mechanisms is like a single unit.
You will have to unscrew it all and place each component in a bucket with mineral spirits or any other grease solvent. Even kerosene will work. Leave the parts in the degreaser for at least 3 days, you will see how the old grease will dissolve and color the degreaser. Air dry it (presurized air). Repeat the cleaning (degreasing) if it still looks greasy. After you see each part is clean and the nickel chorme is gleaming and sparkling, grease it up (with the same type of microtome grease you would use for any modern microtome; I always used the Carl Jung light oil No. 405; Leica has also a very fine light oil,called "Schlitten-bahnenol Nr. 601".
The feeding trigger has to be lubricated with light oil, because its return to vertical is caused by gravity, and cannot be stuck in any way.
The screw for the feeding mechanism has to receive solid grease (Jelly lubrican), I always used the Lipshaw No.294
This type of maintenance I used to do, at least annually.
Enjoy your cleaning!
René J.
Caroline Bass <cbass <@t> wfubmc.edu> wrote:
Hi guys,
So I've inherited an American Optical 860 sliding microtome. I consider
this quite a find since it is my all time favorite for slicing fixed brains.
However, it has been packed away for quite a while and seems to be a bit
gummed up and is in parts. Does anyone have suggestions on how to get this
back into working order. I don't suppose there is a manual floating around?
Any general suggestions for degreasing to remove the stick points. How
about a location for spare parts, particularly dry ice stages and maybe a
disposable blade holder?
Any and all suggestions are appreciated.
Thanks,
Caroline Bass
_______________________________________________
Histonet mailing list
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
---------------------------------
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------------------------------
Message: 24
Date: Wed, 12 Sep 2007 08:39:55 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] QC labeling
To: Marcia Funk <FUNKM <@t> mercyhealth.com>,
histonet <@t> lists.utsouthwestern.edu
Message-ID: <531579.50543.qm <@t> web61222.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Marcia:
Short answer, yes!
I used to keep a file for each HT, and in it everything was written down: the good, the bad, the uggly; trainings, commendations, attendance, you name it.
It was updated everytime anything worth mentioning took place, from commendations from the pathologists for good sections, staining, whatever; as well as any mistakes, not only dealing with mislabelling, but with poor quality of sections, floaters, as I wrote, every thing.
Come the evaluation day all those things, good and bad, all documented AT THE MOMENT THEY HAPPENED, not after the fact, we weighed and most certainly affected the evaluation, either positively or negatively.
Once that evaluation was completed, signed, agreed or disagreed, that annual file was incorporated to the personal file, and a NEW file was opened for the next evaluation period.
Since all issues were addressed immediately they happened or were found, I never had a single case of a HT saying: "I did not know about that", they knew and received retraining if necessary.
René J.
Marcia Funk <FUNKM <@t> mercyhealth.com> wrote:
Histo Friends,
I have a few questions I would like to see how labs are following through with labeling errors?
Case number placed on slide during sectioning. Slide is then mislabeled handed off to pathologist.
Pathologist discovers the error. Slide is labeled incorrectly.
Wrong label placed on slide during labeling. Pathologist discovers mislabeld slide
Errors are place on QC log at the end of day. These are errors that are caught in the department
by the tech or pathologist. Do most labs keep a log with errors by tech and how many errors
impact there eval ?
I would appreciate your imput.
Mrf
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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------------------------------
Message: 25
Date: Wed, 12 Sep 2007 11:45:47 -0500
From: mari.ann.mailhiot <@t> leica-microsystems.com
Subject: Re: [Histonet] AO 860 sliding microtome
To: Caroline Bass <cbass <@t> wfubmc.edu>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OFA173B95A.D4F2195B-ON86257354.005C0C53-86257354.005C1527 <@t> leica-microsystems.com>
Content-Type: text/plain; charset=US-ASCII
Hi Caroline
Please contact me here at Leica about the AO860 sliding microtome.
Rene has offered an excellent clean application for this unit. Thanks
Renee.
Best Regards
Mari Ann Mailhiot BA HT ASCP
Application Specialist
Leica Technical Assistance Center
800 248 0123 x7267
847 236 3063 fax
mari.ann.mailhiot <@t> leica-microsystems.com
www.leica-microsystems.com
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