[Histonet] co-localisation within a cell using DAB and Vulcan Fast Red

Cathy Malcontenti-Wilson cmalc <@t> unimelb.edu.au
Wed Sep 12 00:28:32 CDT 2007


Dear Histonetters,
Does anyone have experience with co-localisation of the chromogens DAB and 
Vulcan Fast Red in an immunohistochemical stain? I have just done a double 
stain and I would like to know what I should I expect if the two antigens I 
have stained are co-localised in the one cell? Would it to look red or 
orange or what??
Much appreciated,

Thanks
Cathy Malcontenti-Wilson




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