[Histonet] toluidine staining faded!

[John Kiernan]

Air drying before mounting is wrong because you get innumerable tiny bubbles within the section, which are held in place by the resinous mounting medium. The section looks grey and opaque. The slide must be wet with xylene before you apply the mounting medium and coverslip. You can often air-dry sections instead of dehydrating with alcohol, but xylene (or equivalent) before coverslipping is a must.

John Kiernan
Anatomy, UWO
London, Canada
---
----- Original Message -----
From: Moran Elishmereni <moran.elish <@t> gmail.com>
Date: Sunday, September 9, 2007 10:02
Subject: [Histonet] toluidine staining faded!
To: histonet <@t> lists.utsouthwestern.edu

> Hello histonetters,
> 
> I stained some murine skin sections for toluidine blue, and mast 
> cells were
> very easy to detect, however, after a few days the staining 
> seemed to have
> faded. Following the tol blue staining, I briefly put the 
> sections in
> alcohol (95%, 100%, 100%), then in xylene substitute, then air 
> dryed and
> mounted with mounting media. What did I do wrong? I am very new
> at histology, so forgive my simple question...
> 
> Thanks,
> Moran
> 
> 
> -- 
> Moran Elishmereni
> 
> Department of Pharmacology and Experimental Therapeutics
> School of Pharmacy, Faculty of Medicine
> The Hebrew University of Jerusalem
> POB 12065
> Jerusalem 91120, ISRAEL
> Tel: 972-2-675-8746
> Fax: 972-2-675-8144
> Email: moran.elish <@t> gmail.com
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