[Histonet] toluidine staining faded!
moran.elish <@t> gmail.com
Sun Sep 9 09:01:10 CDT 2007
I stained some murine skin sections for toluidine blue, and mast cells were
very easy to detect, however, after a few days the staining seemed to have
faded. Following the tol blue staining, I briefly put the sections in
alcohol (95%, 100%, 100%), then in xylene substitute, then air dryed and
mounted with mounting media. What did I do wrong? I am very new
at histology, so forgive my simple question...
Department of Pharmacology and Experimental Therapeutics
School of Pharmacy, Faculty of Medicine
The Hebrew University of Jerusalem
Jerusalem 91120, ISRAEL
Email: moran.elish <@t> gmail.com
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