[Histonet] toluidine staining faded!

[Moran Elishmereni]

Hello histonetters,

I stained some murine skin sections for toluidine blue, and mast cells were
very easy to detect, however, after a few days the staining seemed to have
faded. Following the tol blue staining, I briefly put the sections in
alcohol (95%, 100%, 100%), then in xylene substitute, then air dryed and
mounted with mounting media. What did I do wrong? I am very new
at histology, so forgive my simple question...

Thanks,
Moran


-- 
Moran Elishmereni

Department of Pharmacology and Experimental Therapeutics
School of Pharmacy, Faculty of Medicine
The Hebrew University of Jerusalem
POB 12065
Jerusalem 91120, ISRAEL
Tel: 972-2-675-8746
Fax: 972-2-675-8144
Email: moran.elish <@t> gmail.com