[Histonet] protocol for clearing tissue!

[John Kiernan]

The clearing agent with the highest refractive index is benzyl benzoate (1.57). It provides glass-like transparency. Methyl salicylate (1.54) is just as good, but the strong wintergreen smell can get a bit tiresome. Methyl benzoate (1.51) is also used for this purpose, but it has a really unpleasant odour so I don't recommend it.

If the specimens do not have to be kept for a long time, xylene (R.I. 1.50) might be good enough.

John Kiernan
Anatomy, UWO
London, Canada
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----- Original Message -----
From: "Vest, Luther" <lfvest <@t> vt.edu>
Date: Thursday, September 6, 2007 13:51
Subject: [Histonet] protocol for clearing tissue!
To: histonet <@t> lists.utsouthwestern.edu

> Dear histonet:
> 
> One of the pathology residents in our department has asked me to post
> this question.
> 
>  
> 
> I am in search of a protocol for clearing tissue. This technique 
> is used
> commonly in vascular research to visualize blood vessels. I want 
> to use
> it to clear mouse lung tissue that contains blue-stained
> (B-galactosidase activity) tumor nodules. Any suggestions?
> 
> Thanks
> 
>  
> 
> Luther Vest (HT ASCP)
> 
> VA-MD Regional College of Veterinary Medicine
> 
> Duckpond Dr. Phase III
> 
> Blacksburg, Va 24061-0443
> 
>  
> 
>  
> 
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