[Histonet] Dimedone PAS

[Hawkins, Hal K.]

For preservation of glycogen, it's important to keep the specimen cold
during fixation.


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Geoff
McAuliffe
Sent: Friday, September 07, 2007 9:05 AM
To: Denise Piontek
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Dimedone PAS

Buffered formalin or Bouin's work well for glycogen demonstration but an

alcoholic fixative (alcoholic Bouin or formalin-alcohol-acetic acid) 
will almost certainly, be better.

Geoff

Denise Piontek wrote:
> Is anyone out there familiar with this staining method to isolate
glycogen? How about alternate fixation methods associated with the
stain? Advice and references are greatly appreciated,
>  
> Denise Bland-Piontek, HTL(ACSP)CTBS(AATB)
> NIBRI
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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