[Histonet] RE:Acetone fixation for immunofluorescence protocols

[James S.]

Mauricio,

You didn't say what your problem was - is it the integrity of your
sections or the fluorescence staining?

I always use acetone fixation for mouse tissues for immunofluorescence
and havent had any problems with the labelling however I have had
problems on and off with the integrity of the sections especially after
long labelling procedures. To combat this I make sure the acetone is
'dry' (I use molecular sieves), I dry the sections for at least 1hr
(sometimes overnight) after cutting and try to keep incubation times to
a minimum. I still occasionally have problems with the sections
disintegrating during labelling so I'd be interested to hear anyone
elses comments!

Sonya