[Histonet] MMP9

[Andi Kappeler]

Hi Patsy

no idea, which clone Abcam is selling. We had reasonably good results (on 
human FFPE tissue) with clone 56-2A4, originally from Oncogene, now from 
Calbiochem (http://www.merckbiosciences.co.uk/Products/pds.asp?catno=IM37). 
We used it at 1 ug Ig/ml after HIER in microwave with a somewhat exotic 
retrieval buffer (100 mM Tris, 5% urea, pH 9.5). We had tried a different 
clone before (15W2) without success. Hope this helps.
Andi Kappeler
Institute of Pathology, University of Bern, Switzerland


----- Original Message ----- 
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, September 06, 2007 6:31 PM
Subject: [Histonet] MMP9


> Does anyone have experience with MMP9 ms monoclonal antibody from Abcam on
> ffpe tissue they could possibly share with me.  This one IHC is driving me
> crazy.
>
> Thank you,
>
> Patsy
>
>
>
> Patsy Ruegg, HT(ASCP)QIHC
>
> IHCtech, LLC
>
> 12635 Montview Blvd. Ste.215
>
> Aurora, Colorado 80045
>
> Phone: 720-859-4060
>
> Fax: 720-859-4110
>
> pruegg <@t> ihctech.net
>
> www.ihctech.net
>
> www.ihcrg.org
>
>
>
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