[Histonet] IHC staining weaker in - P.S.

[Dawson, Glen]

I concur with Paul on this one...sounds like a fixation problem.

Glen Dawson  
IHC Manager
Milwaukee, WI

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Monfils,
Paul
Sent: Thursday, September 06, 2007 11:38 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC staining weaker in - P.S.


P.S. Of course, even with a faster penetrating fixative like formalin, the same artifact can be produced simply by insufficient time in the fixative to allow full penetration.  Are you seeing this artifact mostly in larger tissue sections?
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