[Histonet] damaged cartilage

[Liz Chlipala]

What type of tissue are you looking at?  Why don=92t you just stain with =
toluidine blue or safranin O.  Both of these stain proteoglycan, areas =
of cartilage damage will show loss of staining. There are also pathology =
parameters such as fibrillation, loss of chondrocytes and chondrocyte =
cloning that are characteristic of cartilage damage.  If you feel you =
need to use a IHC stain, you could try collagen II.  Articular cartilage =
should stain positive for collagen II.  But I would initally try the =
special stains.

Liz=20

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 735-5001
fax (303) 735-3540
liz <@t> premierlab.com
www.premierlab.com
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Ship to Address:
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Premier Laboratory, LLC
University of Colorado at Boulder
MCDB, Room A3B40
Boulder, CO 80309

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu =
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Walters, =
Katherine S
Sent: Thursday, September 06, 2007 10:46 AM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] damaged cartilage

Does anyone know of a good antibody for damaged cartilage?  I found one =
very expensive one in Canada, but it is cost prohibitive.

=20

Thanks,

=20

Kathy Walters=20

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Katherine Walters
Histology Director
Central Microscopy Research Facility
University of Iowa
85 Eckstein Medical Research Building
Iowa City, Iowa 52242-1101
phone:  (319) 335-8142
fax: (319) 384-4469
katherine-walters <@t> uiowa.edu=20

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