[Histonet] Xylene

[Kemlo Rogerson]

Can somebody tell me if there are any effects on tissue if there is
prolonged processing time in xylene? 

Jason McGough HT(ASCP)
Clinical Laboratory of the Black Hills
Account Representative - Anatomic Pathology 
2805 5th Street
Rapid City, SD 57701
605-343-2267
jmcgough <@t> clinlab.com

Classically it is said to harden, plus more lipids could be removed.
Personally I'm equivocal about that thought; if properly fixed prior to
processing I would have thought the hardening effects of xylene were
much less than that of the coagulant fixative ethanol. If you do have
hard tissue then there is a restorative fluid one can use which I think
has oil of cedarwood in it but I don't know the formula off hand. I know
it works cos when I was a pup I used it sometimes.


Kemlo Rogerson
Pathology Manager
DD   01934 647057 or extension 3311
Mob 07749 754194; Pager 07659 597107;


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