[Histonet] Alcain blue vs. Movats for GAG staining

[Geoff McAuliffe]

Hi Albert:

    As Kim mentioned fixing with an alcoholic fixative (methacarn or 
formalin-alcohol-acetic acid) will improve retention of GAG which should 
be plentiful in arteries. You can also put Safranin O (J. Ultrastructure 
Res. 54:451-460, 1976) or tannic acid (J. Histochem. Cytochem. 
31:783-790, 1983) in the fixative to increase retention.

Geoff

AGrobe2555 <@t> aol.com wrote:
> Hello All,
> Does anyone have a handle how GAG staining by Movats Pentachrome compares  to 
> low pH Alcian blue staining?  I was asked to stain for GAGs in  vascular 
> sections (blood vessels and grafts), and was wondering which would be  the best 
> method (please suggest other methods).  I suspect there won't  be much staining 
> for GAGs anyway.  Please correct me if this is  incorrect, as I have little 
> experience in this area.
> Thanks,
> Albert 
>
>
>
> ************************************** Get a sneak peek of the all-new AOL at 
> http://discover.aol.com/memed/aolcom30tour
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>   


-- 
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 
mcauliff <@t> umdnj.edu
**********************************************