[Histonet] Alcain blue vs. Movats for GAG staining

[Kim Merriam]

In a past life, I used to use Alcian Blue for GAG staining (I can't remember if it was pH 1 or pH 2.5); we used to fix the tissues in Methacarn and that made a big difference in GAG retention (vs NBF).

Good luck,
Kim
 
Kim Merriam, MA, HT(ASCP)
Cambridge, MA



----- Original Message ----
From: "AGrobe2555 <@t> aol.com" <AGrobe2555 <@t> aol.com>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Tuesday, September 4, 2007 4:32:30 PM
Subject: [Histonet] Alcain blue vs. Movats for GAG staining


Hello All,
Does anyone have a handle how GAG staining by Movats Pentachrome compares  to 
low pH Alcian blue staining?  I was asked to stain for GAGs in  vascular 
sections (blood vessels and grafts), and was wondering which would be  the best 
method (please suggest other methods).  I suspect there won't  be much staining 
for GAGs anyway.  Please correct me if this is  incorrect, as I have little 
experience in this area.
Thanks,
Albert 



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