[Histonet] Staining tissue in Embed 812 resin with Hematoxylin and Eosin?

[Geoff McAuliffe]

Hi Larry:

    There is, in my opinion, no easy or good, way to do this. If there 
were, it would probably be in the archives. There were a lot of methods 
papers published in the late 1960's and into the early 1980's on various 
ways to get and H&E picture of glut. fixed, osmicated, plastic embedded 
tissue. The few I looked into seemed like way more trouble than the 
results demonstrated.

Geoff


Lawrence Mason wrote:
> I have some archived mouse tissues in Embed 812 resin.  Is it possible to stain these tissues with hematoxylin and eosin?  I was unable to find any relevant info in the archives.
>  
> Thanks,
> Larry Mason
> Wyeth Research
> Cambridge, MA
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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