[Histonet] curious about soaking paraffin blocks.

[Tarango, Mark]

If you process animal tissue on a human processing schedule, it
definitely will come out very dry.

I remember trying to cut fish brains from some fish that washed ashore a
few years ago.  As soon as the tissue hit the knife, it all turned to
powder.  I had to let the blocks float on the warm waterbath, sit in
soapy ammonia water, and huff and puff all over to get a nice section. 

Also, a researcher here made some antibodies against a synthetic peptide
of a nuclear transcription factor that he is studying.  The sequence he
used was the same for both the human and the mouse versions of the
protein; however, I couldn't get the antibody to work on mouse tissue,
until I modified the processing protocol such that the tissue came out
looking like human tissue (which just took cutting the dehydration
time).  

I just think animal tissue doesn't need to be processed as long as human
tissue does.  Maybe we humans drink more water or something.


Mark Adam Tarango HT(ASCP)
Histology & IHC Supervisor
Nevada Cancer Institute
One Breakthrough Way
Las Vegas, NV  89135
Direct Line (702) 822-5112
Treo (702) 759-9229
Fax (702) 939-7663

  


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kaliko,
Bonnie
Sent: Friday, October 26, 2007 6:28 AM
To: Kemlo Rogerson; Weems, Joyce; Mike Pence; Cheri Miller; Smith Wanda;
Anna K. Schultz; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] curious about soaking paraffin blocks.

Kemlo, you obviously do not work with animal tissue which is VERY dry.
It has nothing to do with poor fixation or over dehydration. Liver,
bone, spleen, muscle is impossible to cut unless it is placed face down
on ice.  If you ever try cutting animal tissue and manage to get a good
section without soaking it, then you should apply for a patent
application.

-----Original Message-----
From: Kemlo Rogerson [mailto:Kemlo.Rogerson <@t> waht.swest.nhs.uk] 
Sent: Friday, October 26, 2007 7:24 AM
To: Weems, Joyce; Kaliko, Bonnie {Nonc~Nutley}; Mike Pence; Cheri
Miller; Smith Wanda; Anna K. Schultz; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] curious about soaking paraffin blocks.

"The key to the perfect section after soaking is not to leave the block
so long that the tissue is damaged. The block then has to be faced to
the exact spot that the perfect section can be taken - not where the
tissue is still too soaked or after you've moved past that place again.
Once again this part has to do with perception and experience."

Helpful.... so obviously a way of answering poor fixation and/ or
processing; sorry but I never came across this in the UK. Do any of you
Brits do this?

Kemlo Rogerson
Pathology Manager
DD   01934 647057 or extension 3311
Mob 07749 754194; Pager 07659 597107;
 
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"EMF <nvcancer.org>" made the following annotations.
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