AW: [Histonet] curious about soaking paraffin blocks.

[Gudrun Lang]

Linda, thank you for explaining. In Austria, we use coolplates. These tend
to get a "snowcoat" after a few hours. The blocks sit in the more or less
dry snow (and wait for their fate). To soften the tissue, if it is too
brittle, we blow at the surface of the block (warm and humid). That works
the most times.

Gudrun Lang
 
Biomed. Analytikerin
Histolabor
Akh Linz
Krankenhausstr. 9
4020 Linz
+43(0)732/7806-6754
-----Ursprüngliche Nachricht-----
Von: Sebree Linda A. [mailto:LSebree <@t> uwhealth.org] 
Gesendet: Freitag, 26. Oktober 2007 18:06
An: gu.lang <@t> gmx.at; histonet <@t> lists.utsouthwestern.edu
Betreff: RE: [Histonet] curious about soaking paraffin blocks.

Our histotechs cool faced blocks on ice that is frozen in plastic
storage containers.  There is usually some melted ice water on top of
the ice block so in essence the blocks are cooled and soaked at the same
time.

Linda Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
A4/204-3224
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gudrun
Lang
Sent: Friday, October 26, 2007 11:03 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: AW: [Histonet] curious about soaking paraffin blocks.


To start at the beginning again. Do the US-histotechs cool the blocks on
a
coolplate and subsequently soak them in ice-water or ammoniacal water?


Gudrun Lang
 
Biomed. Analytikerin
Histolabor
Akh Linz
Krankenhausstr. 9
4020 Linz
+43(0)732/7806-6754


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