[Histonet] soaking paraffin blocks.

[Truscott, Tom]

Hi All, The art of histology would take more than a lifetime to perfect
for every tissue. In my limited experience, I've come to believe that
poor fixation is often blamed when poor processing is the real culprit.
Poor processing will also cause tissues to "explode" on a waterbath.
Optimal fixation and optimal processing can differ for each tissue and
each species, so when you perform histology on a mix of tissues and
species you try to find the average optimal protocol or two that you can
fit into your schedule, then try to overcome problems with chilling or
soaking or lower waterbath temps or whatever other tricks you've learned
from experience or the histonet. Tom Truscott USDA Pullman, WA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Smith,
Allen
Sent: Friday, October 26, 2007 4:53 AM
To: Kemlo Rogerson
Cc: histonet <@t> pathology.swmed.edu
Subject: RE: [Histonet] soaking paraffin blocks.

Yes, soaking the faced block is a cure for poor processing, but it
works.  I
find that holding a moist ice cube against the block face for about 30
seconds does wonders for a poorly processed block.

Allen A. Smith, Ph.D.
Professor of Anatomy
Barry University School of Graduate Medical Sciences
    Podiatric Medicine and Surgery
Miami Shores, Florida  33161


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kemlo
Rogerson
Sent: Friday, October 26, 2007 7:24 AM
To: Weems, Joyce; Kaliko, Bonnie; Mike Pence; Cheri Miller; Smith Wanda;
Anna K. Schultz; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] curious about soaking paraffin blocks.

"The key to the perfect section after soaking is not to leave the block
so long that the tissue is damaged. The block then has to be faced to
the exact spot that the perfect section can be taken - not where the
tissue is still too soaked or after you've moved past that place again.
Once again this part has to do with perception and experience."

Helpful.... so obviously a way of answering poor fixation and/ or
processing; sorry but I never came across this in the UK. Do any of you
Brits do this?

Kemlo Rogerson
Pathology Manager
DD   01934 647057 or extension 3311
Mob 07749 754194; Pager 07659 597107;
 
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