[Histonet] background staining after DAB

[sheila adey]

Hi Rene,
Could you explain to me why having a low concentration of antibody would give background staining? I am having trouble only on my negatives with background. (we use AEC). 
Sheila Adey HT MLTPort Huron HospitalMichigan> Date: Thu, 25 Oct 2007 11:22:49 -0700> From: rjbuesa <@t> yahoo.com> To: dmccaig <@t> ckha.on.ca; histonet <@t> lists.utsouthwestern.edu> Subject: Re: [Histonet] background staining after DAB> CC: > > I would check the antibody dilution, it is probably too low.> René J.> > Diana McCaig <dmccaig <@t> ckha.on.ca> wrote:> Hi> We are using the Biocare IHC protocol and using Fisher superfrost plus> slides (due to decloaking). When doing the double stain, there is a lot> of background stain from the DAB. We do wash well so that is not the> problem. I am wondering if is the charge on the slides. I have checked> the pH of all the solutions and they are okay. Any ideas. The stain is> readable, but does not look too nice.> thanks> Diana> _______________________________________________> Histonet mailing list> Histonet <@t> lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > __________________________________________________> Do You Yahoo!?> Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > _______________________________________________> Histonet mailing list> Histonet <@t> lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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