[Histonet] curious about soaking paraffin blocks.

[Kaliko, Bonnie]

Since we process every organ of rat, mouse, dog, rabbit and monkey on
many animals, processing by hand would be archaic.  The amount of time
each species is processed depends on the tissue size. Mouse having the
shortest processing schedule.  Monkey is the easiest to section because
it is so close to human tissue.  All tissue is fully fixed in 10% NBF
before processing. 

-----Original Message-----
From: Kemlo Rogerson [mailto:Kemlo.Rogerson <@t> waht.swest.nhs.uk] 
Sent: Friday, October 26, 2007 10:24 AM
To: Kaliko, Bonnie {Nonc~Nutley}; Weems, Joyce; Mike Pence; Cheri
Miller; Smith Wanda; Anna K. Schultz; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] curious about soaking paraffin blocks.

I tended to process until they were processed; some tissue took longer
than others the secret was when they looked clear in xylene. If they
weren't see through I went back to alcohol; obviously NOT an automatic
processing procedure.

Kemlo Rogerson
Pathology Manager
DD   01934 647057 or extension 3311
Mob 07749 754194; Pager 07659 597107;
 
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