[Histonet] curious about soaking paraffin blocks.

[Kaliko, Bonnie]

I would love to find out the processing schedule.

-----Original Message-----
From: Kemlo Rogerson [mailto:Kemlo.Rogerson <@t> waht.swest.nhs.uk] 
Sent: Friday, October 26, 2007 10:16 AM
To: Kaliko, Bonnie {Nonc~Nutley}; Weems, Joyce; Mike Pence; Cheri
Miller; Smith Wanda; Anna K. Schultz; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] curious about soaking paraffin blocks.

"Kemlo, you obviously do not work with animal tissue which is VERY dry.
It has nothing to do with poor fixation or over dehydration. Liver,
bone, spleen, muscle is impossible to cut unless it is placed face down
on ice.  If you ever try cutting animal tissue and manage to get a good
section without soaking it, then you should apply for a patent
application."

I ran a Veterinary Histology Laboratory from a number of years and cut
sections from little birdies to big bad apes; never had the problem you
allude to. Must be the way we process in the UK; or is it the water <g>.


Kemlo Rogerson
Pathology Manager
DD   01934 647057 or extension 3311
Mob 07749 754194; Pager 07659 597107;
 
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