[Histonet] curious about soaking paraffin blocks.

Fri Oct 26 05:46:02 CDT 2007

I find that soaking blocks on a wet tissue at room temperature, as opposed 
to an ice water bath is very helpful to stop haemorrhagic tissues 
shattering.

Piero Nelva
Anatomical Pathology
Monash Medical Centre
Melbourne
Australia
----- Original Message ----- 
From: "Cheri Miller" <cmiller <@t> physlab.com>
To: "'Heckford, Karen - SMMC-SF'" <Karen.Heckford <@t> CHW.edu>; 
<histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, October 26, 2007 2:46 AM
Subject: RE: [Histonet] curious about soaking paraffin blocks.

> Some on the net just like to try and make you feel foolish. I know of
> SEVERAL techs (in diff labs, facilities, states etc.) that soak on 
> occasion
> when they feel it will benefit the cut etc. It's called technique. As we 
> all
> know, not all is equal in histology..........Cheri
>
> Cheri Miller HT ASCP
> Histology Supervisor
> Physicians Laboratory Services, Inc.
> Omaha, NE 68117
> 402 738 5052
>
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> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Heckford,
> Karen - SMMC-SF
> Sent: Thursday, October 25, 2007 10:49 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] curious about soaking paraffin blocks.
>
> I always face my blocks and then place them on the ice facing upwards.  If
> I need to soak any blocks I use ammonia water only for about a minute.  I
> have in the past used Downey fabric softener (the original scent only) 
> this
> works great on big tissue like uterus.  It also makes the lab smell like a
> laundry facility.
> Karen Heckford HT (ASCP) CE
> Lead Histology Technician
> Histology/Pathology Department
> St. Mary's Medical Center
> 450 Stanyan St.
> San Francisco, Ca. 94117
> 415-668-1000 ext. 6167
> Fax: 415-750-8123
> email: kheckfor <@t> chw.edu
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cheri 
> Miller
> Sent: Thursday, October 25, 2007 6:34 AM
> To: 'Smith Wanda'; 'Anna K. Schultz'; histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] curious about soaking paraffin blocks.
>
> My experience shows soaking sometimes facilitates better sectioning it 
> just
> depends on the tissue. When I do soak blocks, the small biopsies I place 
> on
> the ice tissue side up so I don't risk swelling.
> Cheri Miller HT ASCP
> Histology Supervisor
> Physicians Laboratory Services, Inc.
> Omaha, NE 68117
> 402 738 5052
>
> PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. 
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> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Smith 
> Wanda
> Sent: Wednesday, October 24, 2007 10:52 AM
> To: Anna K. Schultz; histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] curious about soaking paraffin blocks.
>
> Anna,
> The danger in leaving faced blocks on the ice too long is it causes the
> tissue to swell.  The part of the tissue that swells will be cut away when
> you start to cut your sections.  This can be bad for tiny biopsy specimens
> or needle core biopsies, because you may cut the tissue away.
> I hope this helps!
> Wanda
>
> Wanda G. Smith, HTL(ASCP)HT
> Trident Medical Center
> Pathology Supervisor
> ph:  843-847-4586
> fx:  843-847-4296
> email:  wanda.smith <@t> hcahealthcare.com
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Anna K.
> Schultz
> Sent: Wednesday, October 24, 2007 11:06 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] curious about soaking paraffin blocks.
>
> Greetings All,
>  I remember reading on here/archives that paraffin blocks should not be
>
> soaked or kept on ice for too long--what is the reasoning behind this?
> I am just plain curious and still fairly new to the field.  I often leave
> blocks on for a long time and this seems to help or at least not hurt.
>
> Thanks,
> Anna
> --
> Anna K. Schultz
> SDRC Core A Research Technician
> Department of Dermatology
> College of Physicians and Surgeons
> Columbia University
> Vanderbilt Clinic 15-211
> 630 West 168^th Street
> New York, NY 10032
> Phone: (212) 305-4954
>
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