[Histonet] curious about soaking paraffin blocks.

[Cheri Miller]

No, I soak the tissue first in warm soapy water( very briefly) then place
them tissue side up on the ice. Otherwise what would be the use???  

Cheri Miller HT ASCP
Histology Supervisor
Physicians Laboratory Services, Inc.
Omaha, NE 68117
402 738 5052

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-----Original Message-----
From: Mike Pence [mailto:mpence <@t> grhs.net] 
Sent: Thursday, October 25, 2007 9:31 AM
To: Cheri Miller; Smith Wanda; Anna K. Schultz;
histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] curious about soaking paraffin blocks.

So if you place the block on the ice tissue up, what are you soaking,
paraffin?

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cheri
Miller
Sent: Thursday, October 25, 2007 8:34 AM
To: 'Smith Wanda'; 'Anna K. Schultz'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] curious about soaking paraffin blocks.


My experience shows soaking sometimes facilitates better sectioning it
just depends on the tissue. When I do soak blocks, the small biopsies I
place on the ice tissue side up so I don't risk swelling. 
Cheri Miller HT ASCP
Histology Supervisor
Physicians Laboratory Services, Inc.
Omaha, NE 68117
402 738 5052

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Smith
Wanda
Sent: Wednesday, October 24, 2007 10:52 AM
To: Anna K. Schultz; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] curious about soaking paraffin blocks.

Anna,
The danger in leaving faced blocks on the ice too long is it causes the
tissue to swell.  The part of the tissue that swells will be cut away
when you start to cut your sections.  This can be bad for tiny biopsy
specimens or needle core biopsies, because you may cut the tissue away.
I hope this helps! Wanda

Wanda G. Smith, HTL(ASCP)HT
Trident Medical Center
Pathology Supervisor
ph:  843-847-4586
fx:  843-847-4296
email:  wanda.smith <@t> hcahealthcare.com
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Anna K.
Schultz
Sent: Wednesday, October 24, 2007 11:06 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] curious about soaking paraffin blocks.

Greetings All,
  I remember reading on here/archives that paraffin blocks should not be

soaked or kept on ice for too long--what is the reasoning behind this?  
I am just plain curious and still fairly new to the field.  I often 
leave blocks on for a long time and this seems to help or at least not
hurt.

Thanks,
Anna
-- 
Anna K. Schultz
SDRC Core A Research Technician
Department of Dermatology
College of Physicians and Surgeons
Columbia University
Vanderbilt Clinic 15-211
630 West 168^th Street
New York, NY 10032
Phone: (212) 305-4954

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