[Histonet] curious about soaking paraffin blocks.

[Smith Wanda]

Anna,
The danger in leaving faced blocks on the ice too long is it causes the
tissue to swell.  The part of the tissue that swells will be cut away
when you start to cut your sections.  This can be bad for tiny biopsy
specimens or needle core biopsies, because you may cut the tissue away.
I hope this helps!
Wanda

Wanda G. Smith, HTL(ASCP)HT
Trident Medical Center
Pathology Supervisor
ph:  843-847-4586
fx:  843-847-4296
email:  wanda.smith <@t> hcahealthcare.com
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Anna K.
Schultz
Sent: Wednesday, October 24, 2007 11:06 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] curious about soaking paraffin blocks.

Greetings All,
  I remember reading on here/archives that paraffin blocks should not be

soaked or kept on ice for too long--what is the reasoning behind this?  
I am just plain curious and still fairly new to the field.  I often 
leave blocks on for a long time and this seems to help or at least not
hurt.

Thanks,
Anna
-- 
Anna K. Schultz
SDRC Core A Research Technician
Department of Dermatology
College of Physicians and Surgeons
Columbia University
Vanderbilt Clinic 15-211
630 West 168^th Street
New York, NY 10032
Phone: (212) 305-4954

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