[Histonet] CD3 antibody

[patsy ruegg]

I would recommend lab vision rab monoclonal for cd3, haven't tried it with
alcohol based fixatives but it works great with formalin fixed tissues for
many species, human, dog, rat, mouse, etc.
Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. #216
Aurora, CO 80010
720-859-4060
fax 720-859-4110
pruegg <@t> ihctech.net
www.ihctech.net 
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Sunday, October 21, 2007 7:00 AM
To: Dahui You; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] CD3 antibody

I used CD-3,T antibody from DAKO (polyclonal rabbit IgG) at 1:200 dilution,
HIER at pH6 incubated for 30 minutes at RT and standard detection systems.
Tonsil as control.
  René J.

Dahui You <dahui_you <@t> yahoo.com> wrote:
  Hello, everyone:
We are trying to identify CD3 positive T cells and/or H1N1 influenza in some
HistoChoice (Amresco) fixed and paraffin embedded mouse lung sections.
Does anybody have experience with these antibodies? Any suggestions on
companies and antibodies?
Thanks in advance and I appreciate your help.
Dahui

Dahui You
Department of Biological Sciences
Louisiana State University
Baton Rouge, LA 70803
Tel:225-578-5960
Fax:225-578-2594



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