[Histonet] sectioning plant seeds

[Perry, Margaret]

We have been asked to section plant seeds that resemble oats.  We have
tried to run them thru formalin and processed the same as our regular
animal tissue.  Then we embedded in wax.  We cannot get a good section.
Has anyone worked with plant seeds?  How do you fix and process them.
Any help will be greatly appreciated. I believe they want to do ISH on
the slides.  

Thanks.

 

Margaret Perry HT (ASCP)

IHC Lab Manager Veterinary Science

Animal Disease Research and Diagnostic Lab

South Dakota State University

Box 2175 North Campus Drive

Brookings SD 57007