[Histonet] Marking pens
Webb, Dorothy L
Dorothy.L.Webb <@t> HealthPartners.Com
Thu Oct 18 09:42:03 CDT 2007
They are from Mercedes Medical and the order part # is:KLI-KPPEN They come 12 to a box for $30. <ove them!! They are manufactured in Norway!!
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Wednesday, October 17, 2007 8:10 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 47, Issue 19
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Today's Topics:
1. Herpes Simplex Virus Controls (Bauer, Karen)
2. PAS for frozens (Mark Elliott)
3. Formalin (Satterfield, Marirose)
4. Shirley Phua is away on course 17-18 October 2007. (Shirley PHUA)
5. Slide labeling pen (Patti Loykasek)
6. RE: Slide labeling pen (sheila adey)
7. RE: Slide labeling pen (Liz Chlipala)
8. RE: Slide labeling pen (Dave Johnson)
9. FUNGI CONTROL BLOCK (Diana McCaig)
10. frozen sections (Atoska Gentry)
11. RE: frozen sections (Weems, Joyce)
12. Re: frozen sections (Rene J Buesa)
13. crazy immuno question - can you restain slides? (Cheryl Cross)
14. Re: crazy immuno question - can you restain slides? (Rene J Buesa)
15. Re: crazy immuno question - can you restain slides? (Cheryl Cross)
16. Slide labeling pens (Patti Loykasek)
17. RE: frozen sections (Mickie Johnson)
18. formalin disposal (Malissa Snyder)
19. C9 antibody (Megan Clarke)
20. cross reaction (Valeria Berno)
21. RE: Formalin (Mickie Johnson)
22. Laser Capture Microdissection and thick rat brain sections
(christina bisgaard)
23. question about charges (Green, Kathy)
24. RE: frozen sections (Sharon Bledsoe)
25. RE: question about charges (Weems, Joyce)
26. RE: Formalin (Weems, Joyce)
----------------------------------------------------------------------
Message: 1
Date: Tue, 16 Oct 2007 12:44:37 -0500
From: "Bauer, Karen" <Bauer.Karen <@t> mayo.edu>
Subject: [Histonet] Herpes Simplex Virus Controls
To: <histonet <@t> lists.utsouthwestern.edu>
Cc: Linda Sebree <LSebree <@t> uwhealth.org>, "Colborn, Lisa K."
<colborn.lisa <@t> mayo.edu>
Message-ID:
<FAEEA161EC98C6439D4892E161BCE18A03A1BD3F <@t> LMMAILVS1.ad.lmmhs.org>
Content-Type: text/plain; charset="iso-8859-1"
Hello to all...
It's a cold, dark, and dreary day in Wisconsin and my husband actually saw snow flakes last week! I'm dreading the winter weather as we approach the holidays. Oh well, on to business...
We are in desperate need of some HSV controls and I'm hoping someone out there has a few I could have. I would be willing to swap with some controls that we have in abundance.
Thanks in advance,
Karen Bauer HT(ASCP)
Histology Supervisor
Luther Hospital
Eau Claire, WI
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Message: 2
Date: Tue, 16 Oct 2007 10:54:06 -0700
From: "Mark Elliott" <MElliott <@t> mrl.ubc.ca>
Subject: [Histonet] PAS for frozens
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4714984E020000D600027301 <@t> mail.mrl.ubc.ca>
Content-Type: text/plain; charset=US-ASCII
Does anyone have a procedure for doing a PAS on frozen tissue?? I assume we need to shorten the times in the various regents, but by how much? Any tips/tricks would be greatly appreciated. We are staining human lung tissue.
Thanks
Mark in dreary Vancouver BC
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Message: 3
Date: Tue, 16 Oct 2007 13:57:21 -0400
From: "Satterfield, Marirose" <Marirose.Satterfield <@t> MercyMemorial.org>
Subject: [Histonet] Formalin
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<BBD46D81DE497443B2FAAF7DB7782FD824D2BB <@t> MMHEXCHVS1.mercymemorial.org>
Content-Type: text/plain; charset="us-ascii"
We are going to be switching to Formalin. Our local water department has informed us that we cannot dump the Formalin down the drain. Could I hear some feed back on how you handle your disposal- waste haulers vs. recycling.
Thanks
Mari
Marirose Satterfield
Histology Supervisor
Mercy Memorial Hospital
------------------------------
Message: 4
Date: Wed, 17 Oct 2007 02:03:41 +0800
From: Shirley PHUA <Shirley_PHUA <@t> hsa.gov.sg>
Subject: [Histonet] Shirley Phua is away on course 17-18 October 2007.
To: histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<OF3E49475C.B0BBEDD7-ON48257376.006336E3-48257376.006336E3 <@t> gems2.gov.sg>
Content-Type: text/plain; charset=US-ASCII
I will be out of the office from 17-10-2007 to 18-10-2007.
I'll be away on course 17-18 October 2007. I'll be back on 19 October 2007.
Pathologists:
I will process your requests when I return. If urgent, please forward your email to Henry_Kyaw <@t> hsa.gov.sg
------------------------------
Message: 5
Date: Tue, 16 Oct 2007 11:22:52 -0700
From: Patti Loykasek <ploykasek <@t> phenopath.com>
Subject: [Histonet] Slide labeling pen
To: histonet <histonet <@t> pathology.swmed.edu>
Message-ID: <C33A4F8C.11968%ploykasek <@t> phenopath.com>
Content-Type: text/plain; charset="US-ASCII"
Hi all. I need some help determining the manufacturer of a slide labeling pen. I received a free sample, and now can't remember where it came from (must be the fumes attacking my brain). The pen is labeled as KP Marker Plus. On our slides it has held up well to deparaffinization reagents, heat pretreatment, etc... Thanks for the help. I'm much chagrined having to post this.
Patti Loykasek BS, HTL, QIHC
PhenoPath Laboratories
Seattle, WA
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unauthorized review, use, disclosure or distribution is prohibited. If
you are not the intended recipient, please contact the sender by e-mail
and destroy all copies of the original message, or you may call PhenoPath
Laboratories, Seattle, WA U.S.A. at (206) 374-9000.
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Message: 6
Date: Tue, 16 Oct 2007 14:34:05 -0400
From: sheila adey <sheila_adey <@t> hotmail.com>
Subject: RE: [Histonet] Slide labeling pen
To: Patti Loykasek <ploykasek <@t> phenopath.com>, histonet
<histonet <@t> pathology.swmed.edu>
Message-ID: <BAY129-W882DF6D3A904ACB4EF2B5939C0 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"
Hi Patti,
We love them to. They are from Mercedes Medical.
Sheila Adey HT MLTPort Huron HospitalMichigan> Date: Tue, 16 Oct 2007 11:22:52 -0700> From: ploykasek <@t> phenopath.com> To: histonet <@t> pathology.swmed.edu> CC: > Subject: [Histonet] Slide labeling pen> > Hi all. I need some help determining the manufacturer of a slide labeling> pen. I received a free sample, and now can't remember where it came from> (must be the fumes attacking my brain). The pen is labeled as KP Marker> Plus. On our slides it has held up well to deparaffinization reagents, heat> pretreatment, etc...> Thanks for the help. I'm much chagrined having to post this.> > > Patti Loykasek BS, HTL, QIHC> PhenoPath Laboratories> Seattle, WA> > > > -------------------------------------------------------------------------> This e-mail message, including any attachments, is for the sole use of> the intended recipients and may contain privileged information. Any > unauthorized review, use, disclosure or distribution is prohibited. If > you are not the intended recipient, please contact the sender by e-mail > _________________________________________________________________
Are you ready for Windows Live Messenger Beta 8.5 ? Get the latest for free today! http://entertainment.sympatico.msn.ca/WindowsLiveMessenger
------------------------------
Message: 7
Date: Tue, 16 Oct 2007 12:53:04 -0600
From: "Liz Chlipala" <liz <@t> premierlab.com>
Subject: RE: [Histonet] Slide labeling pen
To: "Patti Loykasek" <ploykasek <@t> phenopath.com>, "histonet"
<histonet <@t> pathology.swmed.edu>
Message-ID:
<EE33BE5C905A3046A7FF8F58A64C8E4B050817 <@t> server.PremierLab.local>
Content-Type: text/plain; charset="us-ascii"
I thinks it mercedes medical
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 735-5001
fax (303) 735-3540
liz <@t> premierlab.com
www.premierlab.com
Ship to Address:
Premier Laboratory, LLC
University of Colorado at Boulder
MCDB, Room A3B40
Boulder, CO 80309
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patti Loykasek
Sent: Tuesday, October 16, 2007 12:45 PM
To: histonet
Subject: [Histonet] Slide labeling pen
Hi all. I need some help determining the manufacturer of a slide labeling pen. I received a free sample, and now can't remember where it came from (must be the fumes attacking my brain). The pen is labeled as KP Marker Plus. On our slides it has held up well to deparaffinization reagents, heat pretreatment, etc... Thanks for the help. I'm much chagrined having to post this.
Patti Loykasek BS, HTL, QIHC
PhenoPath Laboratories
Seattle, WA
------------------------------------------------------------------------
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This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000.
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------------------------------
Message: 8
Date: Tue, 16 Oct 2007 15:03:31 -0400
From: "Dave Johnson" <djohnson <@t> mercedesmedical.com>
Subject: RE: [Histonet] Slide labeling pen
To: "Liz Chlipala" <liz <@t> premierlab.com>, "Patti Loykasek"
<ploykasek <@t> phenopath.com>, "histonet" <histonet <@t> pathology.swmed.edu>
Message-ID:
<46D38D90616923469B1C86663FC1327402466500 <@t> mailserver.mercedesmedical.com>
Content-Type: text/plain; charset="us-ascii"
Yes, it is indeed Mercedes Medical
Give us a call. 800-331-2716
Dave Johnson
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Liz Chlipala
Sent: Tuesday, October 16, 2007 2:53 PM
To: Patti Loykasek; histonet
Subject: RE: [Histonet] Slide labeling pen
I thinks it mercedes medical
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 735-5001
fax (303) 735-3540
liz <@t> premierlab.com
www.premierlab.com
Ship to Address:
Premier Laboratory, LLC
University of Colorado at Boulder
MCDB, Room A3B40
Boulder, CO 80309
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patti Loykasek
Sent: Tuesday, October 16, 2007 12:45 PM
To: histonet
Subject: [Histonet] Slide labeling pen
Hi all. I need some help determining the manufacturer of a slide labeling pen. I received a free sample, and now can't remember where it came from (must be the fumes attacking my brain). The pen is labeled as KP Marker Plus. On our slides it has held up well to deparaffinization reagents, heat pretreatment, etc... Thanks for the help. I'm much chagrined having to post this.
Patti Loykasek BS, HTL, QIHC
PhenoPath Laboratories
Seattle, WA
------------------------------------------------------------------------
-
This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000.
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------------------------------
Message: 9
Date: Tue, 16 Oct 2007 15:03:47 -0400
From: "Diana McCaig" <dmccaig <@t> ckha.on.ca>
Subject: [Histonet] FUNGI CONTROL BLOCK
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<DCFD9E6A390E294AAF3A2561CD32E5C40460DDAF <@t> ckhamail1.ckha.on.ca>
Content-Type: text/plain; charset="us-ascii"
Does someone have a good fungus control block that demonstrates hyphae? Would be truly appreciated Diana McCaig Histology Lab Chatham Kent Health Alliance Chatham. Ontario N8A 5E1 519-352-6401 (6604)
------------------------------
Message: 10
Date: Tue, 16 Oct 2007 14:54:39 -0500
From: Atoska Gentry <gentras <@t> vetmed.auburn.edu>
Subject: [Histonet] frozen sections
To: Histonet <histonet <@t> pathology.swmed.edu>
Message-ID: <471516FF.8000108 <@t> vetmed.auburn.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
hello, has anyone observed empty space surrounding tissue frozen in
O.C.T. or similar tissue freezing medium after snap freezing? Upon
sectioning samples shipped previously snap frozen to one of our
researchers I noticed space surrounding various areas of the tissue.
Does anyone know what probably caused this? Could this be a factor in
the generation of horrible sections? I suspect the tissue may be moving
when the blade touches it. Is there a know remedy that will not damage
the tissue? Has anyone ever added O.C.T. to already frozen tissues? Any
assistance will be much appreciated. Atoska
--
Atoska S. Gentry, B.S., HT(ASCP)
Research Assistant IV
Scott-Ritchey RSCH Center
College of Vet. Med
Auburn, AL 36849
PH (334) 844-5579
FAX (334) 844-5850
email: gentras <@t> vetmed.auburn.edu
------------------------------
Message: 11
Date: Tue, 16 Oct 2007 15:58:01 -0400
From: "Weems, Joyce" <JWEEMS <@t> sjha.org>
Subject: RE: [Histonet] frozen sections
To: "Atoska Gentry" <gentras <@t> vetmed.auburn.edu>, "Histonet"
<histonet <@t> pathology.swmed.edu>
Message-ID:
<1CD6831EB9B26D45B0A3EAA79F7EBD32048F3F38 <@t> sjhaexc02.sjha.org>
Content-Type: text/plain; charset="utf-8"
Please the tissue in baby powder and it will eliminate the air bubble. You can also add baby powder to the OCT and it will give it more "body" for holding the tissue correctly for freezing. I don't remember where I heard this or learned it... I just know it works! Hope it works for you, j
Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
404-851-7376 - Phone
404-851-7831 - Fax
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Atoska Gentry
Sent: Tuesday, October 16, 2007 3:55 PM
To: Histonet
Subject: [Histonet] frozen sections
hello, has anyone observed empty space surrounding tissue frozen in
O.C.T. or similar tissue freezing medium after snap freezing? Upon
sectioning samples shipped previously snap frozen to one of our
researchers I noticed space surrounding various areas of the tissue.
Does anyone know what probably caused this? Could this be a factor in
the generation of horrible sections? I suspect the tissue may be moving
when the blade touches it. Is there a know remedy that will not damage
the tissue? Has anyone ever added O.C.T. to already frozen tissues? Any
assistance will be much appreciated. Atoska
--
Atoska S. Gentry, B.S., HT(ASCP)
Research Assistant IV
Scott-Ritchey RSCH Center
College of Vet. Med
Auburn, AL 36849
PH (334) 844-5579
FAX (334) 844-5850
email: gentras <@t> vetmed.auburn.edu
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Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc.
------------------------------
Message: 12
Date: Tue, 16 Oct 2007 13:34:22 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] frozen sections
To: Atoska Gentry <gentras <@t> vetmed.auburn.edu>, Histonet
<histonet <@t> pathology.swmed.edu>
Message-ID: <846676.2416.qm <@t> web61211.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Yes, if there is a space beyween the OCT and the tissue, you can "refill" it and freeze it again.
René J.
Atoska Gentry <gentras <@t> vetmed.auburn.edu> wrote:
hello, has anyone observed empty space surrounding tissue frozen in
O.C.T. or similar tissue freezing medium after snap freezing? Upon
sectioning samples shipped previously snap frozen to one of our
researchers I noticed space surrounding various areas of the tissue.
Does anyone know what probably caused this? Could this be a factor in
the generation of horrible sections? I suspect the tissue may be moving
when the blade touches it. Is there a know remedy that will not damage
the tissue? Has anyone ever added O.C.T. to already frozen tissues? Any
assistance will be much appreciated. Atoska
--
Atoska S. Gentry, B.S., HT(ASCP)
Research Assistant IV
Scott-Ritchey RSCH Center
College of Vet. Med
Auburn, AL 36849
PH (334) 844-5579
FAX (334) 844-5850
email: gentras <@t> vetmed.auburn.edu
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
---------------------------------
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------------------------------
Message: 13
Date: Tue, 16 Oct 2007 16:39:04 -0400
From: Cheryl Cross <ccross6032 <@t> aol.com>
Subject: [Histonet] crazy immuno question - can you restain slides?
To: histonet <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <009D9AB1-E76A-45DD-83F1-3ABE7BEE760D <@t> aol.com>
Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed
hi everybody -
I'm going back through some of my earlier attempts at immuno for
cleaved caspase 3; i have a beautiful lesion i ruined :) on
dilutions. is there anyway i can use my newer, better protocol on
this previously stained slide?
seems such a waste :)
thanks for any input!
CC
Cheryl Cross, DVM, Dipl. ACVP
Researcher
University Corporation for Atmospheric Research
College of Veterinary Medicine
University of Tennessee Department of Pathology
2407 River Drive, Room A201
Knoxville, TN 37996-4542
(423) 967-2724
fax: 865-974-5616
ccross <@t> ucar.edu
------------------------------
Message: 14
Date: Tue, 16 Oct 2007 13:58:04 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] crazy immuno question - can you restain
slides?
To: Cheryl Cross <ccross6032 <@t> aol.com>, histonet
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <474958.73016.qm <@t> web61213.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
If the section already has DAB (as it probably does), you cannot eliminate it.
René J.
Cheryl Cross <ccross6032 <@t> aol.com> wrote:
hi everybody -
I'm going back through some of my earlier attempts at immuno for
cleaved caspase 3; i have a beautiful lesion i ruined :) on
dilutions. is there anyway i can use my newer, better protocol on
this previously stained slide?
seems such a waste :)
thanks for any input!
CC
Cheryl Cross, DVM, Dipl. ACVP
Researcher
University Corporation for Atmospheric Research
College of Veterinary Medicine
University of Tennessee Department of Pathology
2407 River Drive, Room A201
Knoxville, TN 37996-4542
(423) 967-2724
fax: 865-974-5616
ccross <@t> ucar.edu
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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Message: 15
Date: Tue, 16 Oct 2007 17:12:20 -0400
From: Cheryl Cross <ccross6032 <@t> aol.com>
Subject: Re: [Histonet] crazy immuno question - can you restain
slides?
To: Rene J Buesa <rjbuesa <@t> yahoo.com>
Cc: histonet <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <980C596F-9F85-4078-B277-D9A8844CB217 <@t> aol.com>
Content-Type: text/plain; charset=ISO-8859-1; delsp=yes; format=flowed
i see - thanks Bonnie and Rene for your input...
The old trial sections have not only a lower dilution that didn't
work, but a completely different antibody. the rest of the protocol
would be the same.
it sounds as though if my old sections were understained it may be
worth a shot.....but no guarantees :) if i'm understanding you
correctly.
thanks!
CC
Cheryl Cross, DVM, Dipl. ACVP
Researcher
University Corporation for Atmospheric Research
College of Veterinary Medicine
University of Tennessee Department of Pathology
2407 River Drive, Room A201
Knoxville, TN 37996-4542
(423) 967-2724
fax: 865-974-5616
ccross <@t> ucar.edu
On Oct 16, 2007, at 4:58 PM, Rene J Buesa wrote:
> If the section already has DAB (as it probably does), you cannot
> eliminate it.
> René J.
>
> Cheryl Cross <ccross6032 <@t> aol.com> wrote:
> hi everybody -
>
> I'm going back through some of my earlier attempts at immuno for
> cleaved caspase 3; i have a beautiful lesion i ruined :) on dilutions.
> is there anyway i can use my newer, better protocol on this previously
> stained slide?
>
> seems such a waste :)
>
> thanks for any input!
>
> CC
>
> Cheryl Cross, DVM, Dipl. ACVP
> Researcher
> University Corporation for Atmospheric Research
> College of Veterinary Medicine
> University of Tennessee Department of Pathology
> 2407 River Drive, Room A201
> Knoxville, TN 37996-4542
> (423) 967-2724
> fax: 865-974-5616
> ccross <@t> ucar.edu
>
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> Need a vacation? Get great deals to amazing places on Yahoo! Travel.
------------------------------
Message: 16
Date: Tue, 16 Oct 2007 14:46:34 -0700
From: Patti Loykasek <ploykasek <@t> phenopath.com>
Subject: [Histonet] Slide labeling pens
To: histonet <histonet <@t> pathology.swmed.edu>
Message-ID: <C33A7F4A.11991%ploykasek <@t> phenopath.com>
Content-Type: text/plain; charset="US-ASCII"
Thanks to all for the answers to my slide labeling pen question. The KP Marker pens are distributed by Mercedes Medical. Unfortunately, the pens are currently on back order. Call & get your name on the list.
Patti Loykasek BS, HTL, QIHC
PhenoPath Laboratories
Seattle, WA
-------------------------------------------------------------------------
This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any
unauthorized review, use, disclosure or distribution is prohibited. If
you are not the intended recipient, please contact the sender by e-mail
and destroy all copies of the original message, or you may call PhenoPath
Laboratories, Seattle, WA U.S.A. at (206) 374-9000.
------------------------------
Message: 17
Date: Tue, 16 Oct 2007 16:13:59 -0700
From: "Mickie Johnson" <mickie25 <@t> netzero.net>
Subject: RE: [Histonet] frozen sections
To: "'Atoska Gentry'" <gentras <@t> vetmed.auburn.edu>, "'Histonet'"
<histonet <@t> pathology.swmed.edu>
Message-ID:
<!&!AAAAAAAAAAAYAAAAAAAAAGSBPkiRJN1Ck0zqFBQmG77CgAAAEAAAANfKP2a9GGJMj0RISsnrATgBAAAAAA==@netzero.net>
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This may be remedied by smearing some OCT to the face of the frozen block. One caution is that this may cause the formation of freezing artifact that looks like small holes next to nuclei or in the center of muscle fibers from the thawing that occurs when adding warm OCT to the frozen block face which then re-freezes causing ice crystals to form. The effect may not go deep in the block though. Good luck.
Mickie
Mickie Johnson, B.S., HTL(ASCP)
Mohs Histology Consulting Services, LLC
& Mohs Lab Staffing
2507 S. Manito Blvd.
Spokane, WA 99203
509-954-7134
Web: www.mohshistotemp.com & www.mohslabstaffing.com
Email: mickie25 <@t> netzero.net
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Atoska Gentry
Sent: Tuesday, October 16, 2007 12:55 PM
To: Histonet
Subject: [Histonet] frozen sections
hello, has anyone observed empty space surrounding tissue frozen in
O.C.T. or similar tissue freezing medium after snap freezing? Upon
sectioning samples shipped previously snap frozen to one of our
researchers I noticed space surrounding various areas of the tissue.
Does anyone know what probably caused this? Could this be a factor in
the generation of horrible sections? I suspect the tissue may be moving
when the blade touches it. Is there a know remedy that will not damage
the tissue? Has anyone ever added O.C.T. to already frozen tissues? Any
assistance will be much appreciated. Atoska
--
Atoska S. Gentry, B.S., HT(ASCP)
Research Assistant IV
Scott-Ritchey RSCH Center
College of Vet. Med
Auburn, AL 36849
PH (334) 844-5579
FAX (334) 844-5850
email: gentras <@t> vetmed.auburn.edu
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 18
Date: Tue, 16 Oct 2007 17:43:26 -0700 (PDT)
From: Malissa Snyder <magicchessa <@t> yahoo.com>
Subject: [Histonet] formalin disposal
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <601608.92888.qm <@t> web50208.mail.re2.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
We actually have a powder neutralizer that we put in the jugs of used formalin and then we are able by state and county to pour it down the drain. Maybe, they will let you do the same.
---------------------------------
Yahoo! oneSearch: Finally, mobile search that gives answers, not web links.
------------------------------
Message: 19
Date: Wed, 17 Oct 2007 13:08:18 +1000
From: "Megan Clarke" <Megan.Clarke <@t> hnehealth.nsw.gov.au>
Subject: [Histonet] C9 antibody
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<47160942020000250001A37A <@t> domainatrix.HAHS.HEALTH.NSW.GOV.AU>
Content-Type: text/plain; charset=US-ASCII
Hi Histonetters
We are looking for an antibody to Complement 9 that can be used for Immunohistochemistry stains on formalin fixed paraffin embedded sections. If there is a company out there that supplies this antibody or someone that already uses this antibody, could you please forward this information to us. A distributor in australia would be helpful. Thanking you in anticipation.
Zenobia Haffajee
HAPS
Newcastle
Australia
------------------------------
Message: 20
Date: Wed, 17 Oct 2007 10:00:04 +0200 (CEST)
From: "Valeria Berno" <valeria.berno <@t> embl.it>
Subject: [Histonet] cross reaction
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <1127.10.251.1.146.1192608004.squirrel <@t> 10.251.1.146>
Content-Type: text/plain;charset=iso-8859-1
Hi,
I am working with a user on neurons culture (embryonic) in order to characterize a list of antibodies for differentiated and undifferentiated markers. Here the problem: either transcription factor (nuclear) and membrane antigens show staining in the nuclei and in the membrane. it seems the antibody sticks on the membrane or enter partially in the nuclei.
The protocol is 4%PFA 10min RT, triton 0.3% 10min, bl0cking in Normal goat serum,and washes in TBS-tween0.1%.
Any suggestions?
Thanks in advance
Valeria
Berno Valeria, PhD
EMBL- Mouse Biology Unit
Campus A. Buzzati-Traverso
Via Ramarini, 32
00015, Monterotondo Scalo (RM)
Italy
Tel: +39 06 90091287
Fax: +39 06 90091406
email: valeria.berno <@t> embl.it
www.embl.it
------------------------------
Message: 21
Date: Wed, 17 Oct 2007 03:52:40 -0700
From: "Mickie Johnson" <mickie25 <@t> netzero.net>
Subject: RE: [Histonet] Formalin
To: "'Satterfield, Marirose'"
<Marirose.Satterfield <@t> MercyMemorial.org>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<!&!AAAAAAAAAAAYAAAAAAAAAGSBPkiRJN1Ck0zqFBQmG77CgAAAEAAAAMcubGHJhkNJr6qw/OIMfMABAAAAAA==@netzero.net>
Content-Type: text/plain; charset="us-ascii"
Creative Waste Solutions in Portland (888)795-8300 has a filtration system which conserves the buffer salts and so greatly extends the useful life of buffered formalin. Give them a call for more details.
Mickie
Mickie Johnson, B.S., HTL(ASCP)
Mohs Histology Consulting Services, LLC
& Mohs Lab Staffing
2507 S. Manito Blvd.
Spokane, WA 99203
509-954-7134
Web: www.mohshistotemp.com & www.mohslabstaffing.com
Email: mickie25 <@t> netzero.net
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Satterfield, Marirose
Sent: Tuesday, October 16, 2007 10:57 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Formalin
We are going to be switching to Formalin. Our local water department has informed us that we cannot dump the Formalin down the drain. Could I hear some feed back on how you handle your disposal- waste haulers vs. recycling.
Thanks
Mari
Marirose Satterfield
Histology Supervisor
Mercy Memorial Hospital
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 22
Date: Wed, 17 Oct 2007 13:28:13 +0200 (CEST)
From: christina bisgaard <christina_bisgaard <@t> yahoo.dk>
Subject: [Histonet] Laser Capture Microdissection and thick rat brain
sections
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <94261.79139.qm <@t> web54410.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Dear Histonetters
I'm in urgent need of help
Does anyone out there have experience with laser capture micro-dissection (Arcturus, Veritas) and thick (60-80µ) rat brain section?
I have problems collecting more than one Dentate Gyrus (DG) subregion from the hippocampal area from rat brain sections onto the macro cap. I have tried both PEN membrane glass slides and FRAME slides.
Could this be due to lack of dehydration? When staining/dehydrating 60µ horizontal sections, I observe severe horizontal tearing of the sections if left for more than 30 sec in each dehydration step. If put in Xylene, they are totally broken. Even at 30 sec we sometimes see "blackness" of parts of the sections.
My staining protocol is: (using this protocol we don't see much horizontal tearing)
1) Directly from -80oC to 75% EtOH, 30sec
2) dH2O, 30sec
3) Stain, Toluidin Blue, 3 min.
4) dH2O, 30sec
5) 50% EtOH, 30sec
6) 75% EtOH, 30sec
7) 96% EtOH, 30 sec
8) 100% EtOH, 30sec
I wish to utilize the cap as much as possible (up to 8 DG per cap) in part for economic reasons. Since the downstream process is 2D gels and protein analysis I need as much tissue from each brain as possible which is why I wish to use thick sections to decrease the number of sections to be microdissected.
I'm kind of stuck right now and running out of ideas. I will appreciate any help and will provide more information concerning our protocol if needed.
Christina Bisgaard, PhD Stud., Center for Psychiatric Research, Denmark
---------------------------------
Få en billig laptop. Se Kelkoos gode tilbud her!
------------------------------
Message: 23
Date: Wed, 17 Oct 2007 08:05:45 -0400
From: "Green, Kathy" <kgreen <@t> hsh.org>
Subject: [Histonet] question about charges
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<E913F833B578464EBC3D917DCDB3F715058FCFE2 <@t> HSHEXCHANGE.hsh.org>
Content-Type: text/plain; charset="us-ascii"
Does anyone have "proof" in regard to the ruling of charging multiple specimens? For example, currently if we get a specimen slip with a uterus, non neoplastic (88307) & an appendix, we can only charge for the "higher" item. Our new director is sure that where he was before they were able to charge for multiple specimens. Our pathologists bill for multiple specimens, but out billing dept. says that we aren't allowed. So if anyone out there does "charge" for multiple specimens like that & have "proof" or something that I can show to billing, I would greatly appreciate it.
Kathy Green, HT
Manager Histology Laboratory
Holy Spirit Hospital
503 N. 21st Street
Camp Hill, PA 17011
kgreen <@t> hsh.org
(717) 763-2930
Blackberry: (717) 370-1726
Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You
------------------------------
Message: 24
Date: Wed, 17 Oct 2007 08:32:49 -0400
From: Sharon Bledsoe <sbledsoe <@t> iupui.edu>
Subject: RE: [Histonet] frozen sections
To: "Weems, Joyce" <JWEEMS <@t> sjha.org>, "Atoska Gentry"
<gentras <@t> vetmed.auburn.edu>, "Histonet" <histonet <@t> pathology.swmed.edu>
Message-ID: <a06240800c33baf3d6e28@[134.68.251.140]>
Content-Type: text/plain; charset="us-ascii" ; format="flowed"
Joyce,
How does the baby powder effect your sample if you have to use
birefringence? Talc polarizes, I have to be careful and use
non-powdered gloves when I section many of our animal tissue as we
are looking for crystals that are identified by birefringence.
Do you note on the slide that you have an additive on a frozen section?
Sharon Bledsoe
At 3:58 PM -0400 10/16/07, Weems, Joyce wrote:
>Content-class: urn:content-classes:message
>Content-Type: text/plain;
> charset="utf-8"
>
>Please the tissue in baby powder and it will eliminate the air
>bubble. You can also add baby powder to the OCT and it will give it
>more "body" for holding the tissue correctly for freezing. I don't
>remember where I heard this or learned it... I just know it works!
>Hope it works for you, j
>
>Joyce Weems
>Pathology Manager
>Saint Joseph's Hospital
>5665 Peachtree Dunwoody Rd NE
>Atlanta, GA 30342
>404-851-7376 - Phone
>404-851-7831 - Fax
>
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Atoska
>Gentry
>Sent: Tuesday, October 16, 2007 3:55 PM
>To: Histonet
>Subject: [Histonet] frozen sections
>
>
>hello, has anyone observed empty space surrounding tissue frozen in
>O.C.T. or similar tissue freezing medium after snap freezing? Upon
>sectioning samples shipped previously snap frozen to one of our
>researchers I noticed space surrounding various areas of the tissue.
>Does anyone know what probably caused this? Could this be a factor in
>the generation of horrible sections? I suspect the tissue may be moving
>when the blade touches it. Is there a know remedy that will not damage
>the tissue? Has anyone ever added O.C.T. to already frozen tissues? Any
>assistance will be much appreciated. Atoska
>
>--
>Atoska S. Gentry, B.S., HT(ASCP)
>Research Assistant IV
>Scott-Ritchey RSCH Center
>College of Vet. Med
>Auburn, AL 36849
>PH (334) 844-5579
>FAX (334) 844-5850
>email: gentras <@t> vetmed.auburn.edu
>
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>Confidentiality Notice ** The information contained in this message
>may be privileged and is confidential information intended for the
>use of the addressee listed above. If you are neither the intended
>recipient nor the employee or agent responsible for delivering this
>message to the intended recipient, you are hereby notified that any
>disclosure, copying, distribution or the taking of any action in
>reliance on the contents of this information is strictly prohibited.
>If you have received this communication in error, please notify us
>immediately by replying to the message and deleting it from your
>computer. Thank you. Saint Joseph's Health System, Inc.
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 25
Date: Wed, 17 Oct 2007 09:02:48 -0400
From: "Weems, Joyce" <JWEEMS <@t> sjha.org>
Subject: RE: [Histonet] question about charges
To: "Green, Kathy" <kgreen <@t> hsh.org>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1CD6831EB9B26D45B0A3EAA79F7EBD32048F3F41 <@t> sjhaexc02.sjha.org>
Content-Type: text/plain; charset="utf-8"
It's in the CPT code guidelines. There are some specimens that need to be "bundled" for charging, but if you have separate specimens that require separate diagnoses, each specimen gets a separate charge.
Yes, you would charge for the uterus - depending on whether it is malignant or not and an 88302 for an incidental appendix. If you have uterus, tubes, and ovaries, there are guidelines about bundling those specimens - sometimes you don't.
There are many good resources for billing. Dennis Padget is a good one. He has good instructions and answers for all questions. Our pathologists have subscribed to his services.
Good luck.
Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
404-851-7376 - Phone
404-851-7831 - Fax
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Green, Kathy
Sent: Wednesday, October 17, 2007 8:06 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] question about charges
Does anyone have "proof" in regard to the ruling of charging multiple specimens? For example, currently if we get a specimen slip with a uterus, non neoplastic (88307) & an appendix, we can only charge for the "higher" item. Our new director is sure that where he was before they were able to charge for multiple specimens. Our pathologists bill for multiple specimens, but out billing dept. says that we aren't allowed. So if anyone out there does "charge" for multiple specimens like that & have "proof" or something that I can show to billing, I would greatly appreciate it.
Kathy Green, HT
Manager Histology Laboratory
Holy Spirit Hospital
503 N. 21st Street
Camp Hill, PA 17011
kgreen <@t> hsh.org
(717) 763-2930
Blackberry: (717) 370-1726
Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You _______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc.
------------------------------
Message: 26
Date: Wed, 17 Oct 2007 09:05:05 -0400
From: "Weems, Joyce" <JWEEMS <@t> sjha.org>
Subject: RE: [Histonet] Formalin
To: <mickie25 <@t> netzero.net>, "Satterfield, Marirose"
<Marirose.Satterfield <@t> MercyMemorial.org>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1CD6831EB9B26D45B0A3EAA79F7EBD32048F3F42 <@t> sjhaexc02.sjha.org>
Content-Type: text/plain; charset="utf-8"
We use the Neutralex system from Sakura. They are certified approved with the state of CA. I rest assured that if CA approves, we are safe to us anywhere!
Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
404-851-7376 - Phone
404-851-7831 - Fax
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Mickie Johnson
Sent: Wednesday, October 17, 2007 6:53 AM
To: 'Satterfield, Marirose'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Formalin
Creative Waste Solutions in Portland (888)795-8300 has a filtration system which conserves the buffer salts and so greatly extends the useful life of buffered formalin. Give them a call for more details.
Mickie
Mickie Johnson, B.S., HTL(ASCP)
Mohs Histology Consulting Services, LLC
& Mohs Lab Staffing
2507 S. Manito Blvd.
Spokane, WA 99203
509-954-7134
Web: www.mohshistotemp.com & www.mohslabstaffing.com
Email: mickie25 <@t> netzero.net
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Satterfield, Marirose
Sent: Tuesday, October 16, 2007 10:57 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Formalin
We are going to be switching to Formalin. Our local water department has informed us that we cannot dump the Formalin down the drain. Could I hear some feed back on how you handle your disposal- waste haulers vs. recycling.
Thanks
Mari
Marirose Satterfield
Histology Supervisor
Mercy Memorial Hospital
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc.
------------------------------
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End of Histonet Digest, Vol 47, Issue 19
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