[Histonet] frozen sections

[Weems, Joyce]

I just mixed it as I needed it and added enough to make a thick paste. Sorry I was not more scientific!
 
Joyce Weems
Pathology Manager
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
404-851-7376 - Phone
404-851-7831 - Fax

-----Original Message-----
From: Robyn Vazquez [mailto:vazquezr <@t> ohsu.edu]
Sent: Wednesday, October 17, 2007 11:40 AM
To: histonet <@t> pathology.swmed.edu; Weems, Joyce; gentras <@t> vetmed.auburn.edu
Subject: RE: [Histonet] frozen sections


Joyce,
How much baby powder do you use, per bottle of OCT?  
 
Thanks
Robyn

>>> "Weems, Joyce" <JWEEMS <@t> sjha.org> 10/16/2007 12:58 PM >>>

Please the tissue in baby powder and it will eliminate the air bubble. You can also add baby powder to the OCT and it will give it more "body" for holding the tissue correctly for freezing. I don't remember where I heard this or learned it... I just know it works! Hope it works for you, j

Joyce Weems
Pathology Manager
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
404-851-7376 - Phone
404-851-7831 - Fax


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[ mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Atoska
Gentry
Sent: Tuesday, October 16, 2007 3:55 PM
To: Histonet
Subject: [Histonet] frozen sections


hello, has anyone observed empty space surrounding tissue frozen in 
O.C.T. or similar tissue freezing medium after snap freezing? Upon 
sectioning samples shipped previously snap frozen to one of our 
researchers I noticed space surrounding various areas of the tissue. 
Does anyone know what probably caused this? Could this be a factor in 
the generation of horrible sections? I suspect the tissue may be moving 
when the blade touches it. Is there a know remedy that will not damage 
the tissue? Has anyone ever added O.C.T. to already frozen tissues? Any 
assistance will be much appreciated. Atoska

-- 
Atoska S. Gentry, B.S., HT(ASCP)
Research Assistant IV
Scott-Ritchey RSCH Center
College of Vet. Med
Auburn, AL 36849
PH (334) 844-5579
FAX (334) 844-5850
email: gentras <@t> vetmed.auburn.edu


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Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc.