[Histonet] frozen sections

[Atoska Gentry]

hello, has anyone observed empty space surrounding tissue frozen in 
O.C.T. or similar tissue freezing medium after snap freezing? Upon 
sectioning samples shipped previously snap frozen to one of our 
researchers I noticed space surrounding various areas of the tissue. 
Does anyone know what probably caused this? Could this be a factor in 
the generation of horrible sections? I suspect the tissue may be moving 
when the blade touches it. Is there a know remedy that will not damage 
the tissue? Has anyone ever added O.C.T. to already frozen tissues? Any 
assistance will be much appreciated. Atoska

-- 
Atoska S. Gentry, B.S., HT(ASCP)
Research Assistant IV
Scott-Ritchey RSCH Center
College of Vet. Med
Auburn, AL 36849
PH (334) 844-5579
FAX (334) 844-5850
email: gentras <@t> vetmed.auburn.edu