[Histonet] CRYOSTAT TROUBLESHOOTING

[Bernice Frederick]

I have found that the reason the tissue falls off from a precooled chuck,
such as leaving them premade and in the cryostat overnight, is because of
the defrost cycle we all have set to run during the night. As a result, we
keep the chucks in the cryostat un made and only make them in the morning.
The chucks can also be placed in the cryostat first thing to cool down and
then the OCT added etc.
Bernice

Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bonner,
Janet
Sent: Tuesday, October 09, 2007 1:12 PM
To: Matt Bancroft; Sebree Linda A.; mstone <@t> cmhlink.org;
histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] CRYOSTAT TROUBLESHOOTING

     We also use the warm chucks.  Without exception, the OCT chunks off the
chuck when the chuck is pre-cooled.  We have chucks of all ages and stages
of use.  We only use the large chucks and clean them in a virex soak and a
wire brush.  Both of these last specifications may be factors! 
     For those of you who precool your chucks, what size/type of chuck are
you using and what do you clean them with?  The chucks come out so much
neater if the chuck is cooler before the OCT is applied!
                             Thank you!    Janet 
 

________________________________

From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Matt Bancroft
Sent: Tue 10/9/2007 11:52 AM
To: Sebree Linda A.; mstone <@t> cmhlink.org; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] CRYOSTAT TROUBLESHOOTING



I am a manger of a mohs lab, I use room temp molds to keep the OCT from
popping off the chuck.
  
  Matt Bancroft HT (ASCP)

"Sebree Linda A." <LSebree <@t> uwhealth.org> wrote:
  Marcy,

I was always taught to apply the OCT to a RT chuck, put in the cryostat
and when you start to see the OCT turn white, i.e. the OCT in the
grooves is starting to freeze, add the tissue and weight down with the
cold heat extractor.

Linda Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
A4/204-3224
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
MARCELLYN STONE
Sent: Tuesday, October 09, 2007 9:40 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] CRYOSTAT TROUBLESHOOTING


Hello..

I am looking for some help. The O.C.T. embedded tissue is falling of
the
chuck when we try to section. Does anyone have any ideas to stop this?
We
have tried new O.C.T., cleaning chucks, advancing slower. Please help.
Thanks Marcy

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